Chen Yue, Zhou Min, Liu Juanxiu, Chi Junzhou, Yang Xin, Du Qin, Ren Xiaohua, Tian Kun
Department of Stomatology, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu 610072, China.
Department of Stomatology, Chengfei Hospital, Chengdu 610091, China.
Int Immunopharmacol. 2024 Mar 30;130:111745. doi: 10.1016/j.intimp.2024.111745. Epub 2024 Mar 1.
Autologous tooth grafting is a dental restorative modality based on periodontal ligament healing.Human periodontal ligament stem cells(PDLSCs) are involved in the formation and remodeling of periodontal tissue.Based on previous findings, the proliferation and differentiation of processing cryopreserved periodontal ligament stem cells (PDLSCs) exhibit similarities to those of fresh cells. However, there is evident absorption in the transplanted frozen tooth's roots and bones, with the underlying cause remaining unknown. Granulocyte macrophage colony-stimulating factor(GM-CSF) is named for its produce granulocyte and macrophage precursors from bone marrow precursors, and it also serves as one of the regulatory factors in inflammatory and osteoclast formation. This study aimed to investigate changes in GM-CSF expression in frozen PDLSCs (fhPDLSCs) and evaluate the impact of GM-CSF on PDLSCs with respect to cellular activity and osteogenic ability. The role of GM-CSF in periodontal absorption was further speculated by comparing with IL-1β. The results revealed a significant increase in GM-CSF levels from fhPDLSCs compared to fresh cells, which exhibited an equivalent inflammatory stimulation effect as 1 ng/ml IL-1β. Cell viability also increased with increasing concentrations of GM-CSF; however, the GM-CSF from fhPDLSCs was not sufficient to significantly trigger osteoclastic factors. Considering its interaction with IL-1β and positive feedback mechanism, environments with high doses of GM-CSF derived from fhPDLSCs are more likely to activate osteoclastic responses.Therefore, for frozen tooth replantation, great attention should be paid to anti-inflammation and anti-infection.GM-CSF may serve as a potential therapeutic target for inhibiting periodontal resorption in delayed grafts.
自体牙移植是一种基于牙周膜愈合的牙齿修复方式。人牙周膜干细胞（PDLSCs）参与牙周组织的形成和重塑。基于先前的研究结果，经冷冻保存的牙周膜干细胞（PDLSCs）的增殖和分化与新鲜细胞相似。然而，移植的冷冻牙的牙根和牙槽骨存在明显吸收，其根本原因尚不清楚。粒细胞巨噬细胞集落刺激因子（GM-CSF）因其能从骨髓前体细胞产生粒细胞和巨噬细胞前体而得名，它也是炎症和破骨细胞形成的调节因子之一。本研究旨在探讨冷冻保存的牙周膜干细胞（fhPDLSCs）中GM-CSF表达的变化，并评估GM-CSF对PDLSCs细胞活性和成骨能力的影响。通过与IL-1β比较，进一步推测GM-CSF在牙周吸收中的作用。结果显示，与新鲜细胞相比，fhPDLSCs中GM-CSF水平显著升高，其炎症刺激作用与1 ng/ml IL-1β相当。细胞活力也随着GM-CSF浓度的增加而增加；然而，fhPDLSCs中的GM-CSF不足以显著触发破骨细胞因子。考虑到其与IL-1β的相互作用和正反馈机制，来自fhPDLSCs的高剂量GM-CSF环境更有可能激活破骨细胞反应。因此，对于冷冻牙再植，应高度重视抗炎和抗感染。GM-CSF可能成为抑制延迟移植中牙周吸收的潜在治疗靶点。
