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富含粒细胞巨噬细胞集落刺激生长因子的壳聚糖纳米凝胶可促进人牙髓干细胞的成牙本质细胞分化。

Chitosan nanogels enriched with granulocyte-macrophage colony-stimulating growth factor promote odontoblastic differentiation in human dental pulp stem cells in vitro.

作者信息

Asheghi Bahar, Asadi Khatereh, Gholami Ahmad, Enteghad Maryam, Sadeghi Seyedeh Saba, Firouzi Negin

机构信息

Department of Endodontics, School of Dentistry, Shiraz University of Medical Sciences, Ghasrodasht St, Mehr Ave, Shiraz, 71956-15878, Iran.

Guilan Road Trauma Research Center, Trauma Institute, Guilan University of Medical Sciences, Rasht, Iran.

出版信息

BMC Oral Health. 2025 Jul 1;25(1):965. doi: 10.1186/s12903-025-06185-x.

DOI:10.1186/s12903-025-06185-x
PMID:40597938
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12220100/
Abstract

Nanomaterials and regeneration-inducing microenvironments are key components of innovative regenerative endodontic treatment (RET). This study aimed to assess the odontogenic potential of granulocyte-macrophage colony-stimulating growth factor (GM-CSF) loaded chitosan nanogels (CNgs) on dental pulp stem cell (DPSCs) culture. GM-CSF/CNgs were prepared through the ionic gelation method and then characterized with Fourier transform infrared spectroscopy (FTIR), UV-visible spectrophotometry, dynamic light scattering (DLS), and zeta potential devices. Acridine orange (AO) and 4',6-diamidino-2-phenylindole (DAPI) were used to evaluate cellular morphology and viability. The odontogenic and osteogenic differentiation was determined by quantitative real-time reverse-transcription PCR (qRT-PCR) and scanning electron microscopy (SEM). The physicochemical characterization confirmed that the GM-CSF/CNgs were prepared. The loading efficiency was 82.9 ± 2. Significant biocompatibility and no apparent nuclear fragmentation upon exposure to GM-CSF/CNgs and CNgs were observed. Quantifying the expression of dental pulp regeneration associated with genes including osteocalcin gene (OCN), dentin sialophosphoprotein (DSPP), and dentin matrix protein 1 (DMP1) between GM-CSF/CNgs and control groups was significant (p < 0.001). Morphology of DPSCs in contact with GM-CSF/CsNgs demonstrated odontogenic differentiation. GM-CSF/CNgs promoted a bioinspired drug delivery system (DDS) and induced dental pulp regeneration of DPSCs.

摘要

纳米材料和诱导再生的微环境是创新性牙髓再生治疗（RET）的关键组成部分。本研究旨在评估负载粒细胞-巨噬细胞集落刺激生长因子（GM-CSF）的壳聚糖纳米凝胶（CNgs）对牙髓干细胞（DPSCs）培养的成牙潜能。通过离子凝胶法制备GM-CSF/CNgs，然后用傅里叶变换红外光谱（FTIR）、紫外可见分光光度法、动态光散射（DLS）和zeta电位仪对其进行表征。使用吖啶橙（AO）和4',6-二脒基-2-苯基吲哚（DAPI）评估细胞形态和活力。通过定量实时逆转录聚合酶链反应（qRT-PCR）和扫描电子显微镜（SEM）确定成牙和成骨分化。物理化学表征证实已制备出GM-CSF/CNgs。负载效率为82.9±2。观察到GM-CSF/CNgs和CNgs具有显著的生物相容性，且暴露后无明显的核碎裂。GM-CSF/CNgs组与对照组之间定量分析牙髓再生相关基因包括骨钙素基因（OCN）、牙本质涎磷蛋白（DSPP）和牙本质基质蛋白1（DMP1）的表达具有显著性差异（p＜0.001）。与GM-CSF/CsNgs接触的DPSCs形态显示有成牙分化。GM-CSF/CNgs促进了一种仿生药物递送系统（DDS）并诱导了DPSCs的牙髓再生。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e44/12220100/a9076307d30d/12903_2025_6185_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e44/12220100/ef534e711536/12903_2025_6185_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e44/12220100/c8f4b28d54b3/12903_2025_6185_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e44/12220100/eba211540bd8/12903_2025_6185_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e44/12220100/4f65502132b9/12903_2025_6185_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e44/12220100/7862779e0017/12903_2025_6185_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e44/12220100/a9076307d30d/12903_2025_6185_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e44/12220100/ef534e711536/12903_2025_6185_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e44/12220100/c8f4b28d54b3/12903_2025_6185_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e44/12220100/eba211540bd8/12903_2025_6185_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e44/12220100/4f65502132b9/12903_2025_6185_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e44/12220100/7862779e0017/12903_2025_6185_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e44/12220100/a9076307d30d/12903_2025_6185_Fig6_HTML.jpg

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[1]
Chitosan nanogels enriched with granulocyte-macrophage colony-stimulating growth factor promote odontoblastic differentiation in human dental pulp stem cells in vitro.

BMC Oral Health. 2025-7-1

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[3]
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[6]
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Arch Oral Biol. 2013-9-10

[7]
Glycol chitin-based thermoresponsive hydrogel scaffold supplemented with enamel matrix derivative promotes odontogenic differentiation of human dental pulp cells.

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[8]
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[9]
[Effects of stromal cell-derived factor-1 on proliferation, migration, and odontoblastic differentiation of human dental pulp stem cells].

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[10]
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本文引用的文献

[1]
Trinitroglycerine-loaded chitosan nanoparticles attenuate renal ischemia-reperfusion injury by modulating oxidative stress.

Sci Rep. 2024-12-30

[2]
Trinitroglycerin-loaded chitosan nanogels accelerate angiogenesis in wound healing process.

Int J Biol Macromol. 2024-10

[3]
Trinitroglycerin-loaded chitosan nanogels: Shedding light on cytotoxicity, antioxidativity, and antibacterial activities.

Int J Biol Macromol. 2024-4

[4]
Multiple effects of dose-related GM-CSF on periodontal resorption in deep-frozen grafted teeth: A reverse study.

Int Immunopharmacol. 2024-3-30

[5]
Using chitosan, hyaluronic acid, alginate, and gelatin-based smart biological hydrogels for drug delivery in oral mucosal lesions: A review.

Int J Biol Macromol. 2023-12-1

[6]
Bringing resistance modulation to methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant enterococci (VRE) strains using a quaternary ammonium compound coupled with zinc oxide nanoparticles.

World J Microbiol Biotechnol. 2023-5-11

[7]
Encapsulation of human endometrial stem cells in chitosan hydrogel containing titanium oxide nanoparticles for dental pulp repair and tissue regeneration in male Wistar rats.

J Biosci Bioeng. 2023-4

[8]
Concentrated Growth Factors Combined with Lipopolysaccharide Stimulate the In Vitro Regenerative and Osteogenic Activities of Human Dental Pulp Stem Cells by Balancing Inflammation.

Int J Dent. 2022-12-15

[9]
Characterization of a bioscaffold containing polysaccharide acemannan and native collagen for pulp tissue regeneration.

Int J Biol Macromol. 2023-1-15

[10]
Photocrosslinkable methacrylated gelatin hydrogel as a cell-friendly injectable delivery system for chlorhexidine in regenerative endodontics.

Dent Mater. 2022-9

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