Chen Xinfei, Lu Minya, Wang Yao, Zhang Han, Jia Xinmiao, Jia Peiyao, Yang Wenhang, Chen Jiawei, Song Guobin, Zhang Jianguo, Xu Yingchun
Department of Laboratory Medicine, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, China.
Beijing Key Laboratory for Mechanisms Research and Precision Diagnosis of Invasive Fungal Diseases (BZ0447), Beijing, China.
Antimicrob Resist Infect Control. 2024 Mar 3;13(1):28. doi: 10.1186/s13756-023-01339-4.
Aeromonas hydrophila infections can cause gastrointestinal symptoms such as diarrhea; however, deep infections are rarely reported. Outbreaks of A. hydrophila are reported more frequently in fish, poultry, and snakes than in humans. This study aimed to track clonal relatedness of deep infections caused by A. hydrophila using whole genome sequencing (WGS).
We collected three isolates of A. hydrophila in July 19 to August 29, 2019, from patients that underwent spine surgery. Accurate species identification was performed using whole-genome average nucleotide identity (ANI). Antimicrobial susceptibility testing was performed using a VITEK 2 automated AST-N334 Gram-negative susceptibility card system. Antimicrobial resistance and virulence genes were identified using the Comprehensive Antibiotic Resistance Database and Virulence Factor Database VFanalyzer.
All three isolates were identified as A. hydrophila based on ANI and multilocus sequence typing analysis revealed that A. hydrophila belonged to a novel sequence type (ST1172). All three isolates were susceptible to amikacin and levofloxacin; however, they were resistant to piperacillin/tazobactam, ceftriaxone, cefuroxime, cefoxitin, and imipenem. Isolate 19W05620 (patient 3) showed increased ceftazidime resistance (minimum inhibitory concentration ≥ 64 µg/mL). All three isolates possessed the same chromosomally encoded β-lactamases, including bla (β-lactamase), imiH (metallo-β-lactamase), and bla (AmpC) in plasmids.
Our study validated the transmission of a novel carbapenem-resistant A. hydrophila sequence type (ST1172) in patients that underwent spine surgery. Control measures should be developed to prevent dissemination of A. hydrophila in the hospital setting.
嗜水气单胞菌感染可引起腹泻等胃肠道症状；然而，深部感染鲜有报道。嗜水气单胞菌在鱼类、家禽和蛇类中的暴发报道比在人类中更为频繁。本研究旨在利用全基因组测序（WGS）追踪嗜水气单胞菌引起的深部感染的克隆相关性。
我们于2019年7月19日至8月29日从接受脊柱手术的患者中收集了三株嗜水气单胞菌分离株。使用全基因组平均核苷酸同一性（ANI）进行准确的菌种鉴定。使用VITEK 2自动AST-N334革兰氏阴性药敏卡系统进行药敏试验。使用综合抗生素耐药性数据库和毒力因子数据库VFanalyzer鉴定抗生素耐药性和毒力基因。
根据ANI鉴定，所有三株分离株均为嗜水气单胞菌，多位点序列分型分析显示嗜水气单胞菌属于一种新的序列类型（ST1172）。所有三株分离株对阿米卡星和左氧氟沙星敏感；然而，它们对哌拉西林/他唑巴坦、头孢曲松、头孢呋辛、头孢西丁和亚胺培南耐药。分离株19W05620（患者3）显示头孢他啶耐药性增加（最低抑菌浓度≥64μg/mL）。所有三株分离株都具有相同的染色体编码β-内酰胺酶，包括质粒中的bla（β-内酰胺酶）、imiH（金属β-内酰胺酶）和bla（AmpC）。
我们的研究证实了一种新型耐碳青霉烯嗜水气单胞菌序列类型（ST1172）在接受脊柱手术的患者中的传播。应制定控制措施以防止嗜水气单胞菌在医院环境中传播。
