Benali Jihen, Ben Atitallah Imen, Ghariani Bouthaina, Mechichi Tahar, Hadrich Bilel, Zouari-Mechichi Héla
Laboratory of Biochemistry and Enzymatic Engineering of Lipases, National School of Engineers of Sfax, University of Sfax, PO box 1173, 3038 Sfax, Tunisia.
Department of Chemical Engineering, College of Engineering, Imam Mohammad Ibn Saud Islamic University, IMSIU, 11432 Riyadh, Saudi Arabia.
3 Biotech. 2024 Mar;14(3):93. doi: 10.1007/s13205-024-03937-4. Epub 2024 Mar 1.
Factors, namely pH, laccase-like activity, dyes concentration as well as 1-Hydroxybenzotriazole (HBT) concentration was examined. The results indicated that the maximum decolorization yield and rate reached 98.30 ± 0.10% and 5.84 ± 0.01%/min, respectively for Sirius Blue, and 99.34 ± 0.47% and 5.85 ± 0.12%/min, respectively for Sirius Red after 4 h. The presence of the redox mediator 1-hydroxybenzotriazole (HBT) greatly improved the decolorization levels. The optimum concentrations of HBT, dyes, and laccase were 0.62 mM, 50 mg/L, and 0.89 U/mL respectively at pH 4.58 for both dyes. Phytotoxicity tests using treated and untreated dyes proved that the applied treatment slightly decreased the toxicity of the by-products. However, the germination index (GI) increased from 14.6 to 36.08% and from 31.6 to 36.96% for Sirius Red and Sirius Blue, respectively. The present study focused on the treatment of two recalcitrant azo dyes, namely: Sirius Blue (Direct Blue 71) and Sirius Red (Direct Red 80). The decolorization was performed using cell-free supernatant from culture with high laccase activity. Response surface methodology (RSM) and Box-Behnken design were applied to optimize the decolorization of the two tested dyes. The effect of four.
研究了pH值、漆酶样活性、染料浓度以及1-羟基苯并三唑（HBT）浓度等因素。结果表明，对于天狼星蓝，4小时后最大脱色率和速率分别达到98.30±0.10%和5.84±0.01%/分钟；对于天狼星红，分别达到99.34±0.47%和5.85±0.12%/分钟。氧化还原介质1-羟基苯并三唑（HBT）的存在极大地提高了脱色水平。对于两种染料，在pH 4.58时，HBT、染料和漆酶的最佳浓度分别为0.62 mM、50 mg/L和0.89 U/mL。使用处理过和未处理的染料进行的植物毒性测试证明，所应用的处理略微降低了副产物的毒性。然而，天狼星红和天狼星蓝的发芽指数（GI）分别从14.6%提高到36.08%和从31.6%提高到36.96%。本研究聚焦于两种难降解偶氮染料的处理，即：天狼星蓝（直接蓝71）和天狼星红（直接红80）。使用具有高漆酶活性的培养物的无细胞上清液进行脱色。应用响应面方法（RSM）和Box-Behnken设计来优化两种测试染料的脱色。研究了四个因素的影响。
