Staita Karima, Khmaissa Marwa, Akrout Imen, Greff Stéphane, Ghariani Bouthaina, Turbé-Doan Annick, Lambert Julien, Lomascolo Anne, Albert Quentin, Faulds Craig B, Sciara Giuliano, Zouari-Mechichi Héla, Record Eric, Mechichi Tahar
Laboratoire de Biochimie et de Génie Enzymatique des Lipases, Ecole Nationale d'Ingénieurs de Sfax, Université de Sfax, Sfax 3038, Tunisia.
INRAE, Aix Marseille Univ BBF, Biodiversité et Biotechnologie Fongiques, 13288 Marseille, France.
J Fungi (Basel). 2024 Dec 12;10(12):861. doi: 10.3390/jof10120861.
Antibiotics play a crucial role in human and animal medical healthcare, but widespread use and overuse of antibiotics poses alarming health and environmental issues. Fluoroquinolones constitute a class of antibiotics that has already become ubiquitous in the environment, and their increasing use and high persistence prompt growing concern. Here we investigated a fungal secretome prepared from the white-rot fungus , which is able to effectively degrade the environmentally persistent fluoroquinolone, levofloxacin. We tested various physical-chemical factors such as concentrations of 1-hydroxybenzotriazol (HBT), of enzyme, and of antibiotic, and pH and temperature of the reaction for biotransformation of the antibiotic. We compared the free with the immobilized secretome proteins, and analyzed the collective reaction products for residual activity against (growth inhibition test). We also performed HPLC analysis. The results show that treatment with the free secretome yielded a highest removal efficiency of 50 mg L levofloxacin in the presence of 2.5 mM HBT, whereas the immobilized secretome was only able to degrade 10 mg L levofloxacin with the same concentration of mediator, but presenting the advantage of being reusable.
抗生素在人类和动物医疗保健中发挥着关键作用，但抗生素的广泛使用和过度使用引发了令人担忧的健康和环境问题。氟喹诺酮类是一类在环境中已无处不在的抗生素，其使用量的增加和高持久性引发了越来越多的关注。在此，我们研究了由白腐真菌制备的真菌分泌组，该白腐真菌能够有效降解环境中持久性的氟喹诺酮类药物左氧氟沙星。我们测试了各种物理化学因素，如1-羟基苯并三唑（HBT）、酶和抗生素的浓度，以及反应的pH值和温度，以实现抗生素的生物转化。我们比较了游离分泌组蛋白和固定化分泌组蛋白，并分析了集体反应产物对（生长抑制试验）的残留活性。我们还进行了高效液相色谱分析。结果表明，在存在2.5 mM HBT的情况下，用游离分泌组处理左氧氟沙星的去除效率最高可达50 mg/L，而固定化分泌组在相同浓度的介质下仅能降解10 mg/L的左氧氟沙星，但具有可重复使用的优点。
