Mirhosseini Nazanin, Li Lin, Liu Zhu, Mamas Mamas, Fraser Douglas, Wang Tao
Engineering Building A, 4th Floor Core 1, Department of Engineering for Sustainability, School of Engineering, Faculty of Science and Engineering, The University of Manchester, Manchester, M13 9PL, UK.
Department of Engineering for Sustainability, School of Engineering, The University of Manchester, Manchester, M13 9PL, UK.
Heliyon. 2024 Feb 19;10(5):e26425. doi: 10.1016/j.heliyon.2024.e26425. eCollection 2024 Mar 15.
Complete endothelialisation of coronary stents is an important determinant of future thrombotic complications following coronary stenting. Stent surface texture is an important factor that influences endothelial cell growth. With the emergence of second and third generation coronary stents, is limited comparative data describing endothelial cell growth in contemporary stent platforms, and limited data available on approaches used to rapidly modify the surfaces of commercial coronary stents to improve endothelialisation. In this study we have determined the proliferation of the primary human coronary artery endothelial cells on the commonly used 4 types of commercial coronary stents and found that the inner surface of BioMatrix drug-eluting stents (DES), after eliminating of the polymer and drug coating, had significantly higher endothelial cell proliferation compared to that of other bare metal stents (BMS): Multi-Link8, Integrity and Omega. The surfaces of the 3 types of BMS which are smooth, displayed similar endothelial cell proliferation, suggesting the importance of surface features in manipulating endothelial cell growth. Laser surface texturing was used to create micro/nano patterns on the stents. The laser treatment has significantly increased endothelial proliferation on the inner surfaces of all 4 types of stents, and Multi-Link8 stents displayed the highest (>100%) improvement. The laser textured BioMatrix stents had the highest absolute number of endothelial cells growth. Our results provided useful information in the endothelialisation potential for the commonly used commercial coronary stents and suggested a potential future application of laser surface bioengineering to coronary stents for better biocompatibility of the device.
冠状动脉支架的完全内皮化是冠状动脉支架置入术后未来血栓形成并发症的重要决定因素。支架表面纹理是影响内皮细胞生长的重要因素。随着第二代和第三代冠状动脉支架的出现，关于当代支架平台内皮细胞生长的比较数据有限，并且关于用于快速修饰商业冠状动脉支架表面以改善内皮化的方法的可用数据也有限。在本研究中，我们测定了原代人冠状动脉内皮细胞在常用的4种商业冠状动脉支架上的增殖情况，发现BioMatrix药物洗脱支架（DES）在去除聚合物和药物涂层后的内表面，与其他裸金属支架（BMS）：Multi-Link8、Integrity和Omega相比，具有显著更高的内皮细胞增殖。3种表面光滑的BMS显示出相似的内皮细胞增殖，表明表面特征在调控内皮细胞生长中的重要性。使用激光表面纹理化在支架上创建微/纳米图案。激光处理显著增加了所有4种类型支架内表面的内皮细胞增殖，并且Multi-Link8支架显示出最高（>100%）的改善。激光纹理化的BioMatrix支架具有最高的内皮细胞生长绝对数量。我们的结果为常用商业冠状动脉支架的内皮化潜力提供了有用信息，并提示激光表面生物工程在冠状动脉支架未来的潜在应用，以提高器械的生物相容性。
