Jitsuhiro Akiko, Maeda Tomoki, Ogawa Akiko, Yamada Sayuri, Konoeda Yuki, Maruyama Hiroki, Endo Fuyuaki, Kitagawa Midori, Tanimoto Keishi, Hotta Atsushi, Tsuji Toshikazu
Kirin Central Research Institute, Kirin Holdings Company Limited, 26-1 Muraoka-Higashi 2-Chome, Fujisawa, Kanagawa 251-8555 Japan.
Frontier Research Center for Applied Atomic Science, Ibaraki University, 162-1 Shirakata, Tokai-mura, Naka-gun, Ibaraki 319-1106, Japan.
ACS Omega. 2024 Feb 15;9(8):9803-9812. doi: 10.1021/acsomega.3c10233. eCollection 2024 Feb 27.
Contact-killing antibacterial materials are attracting attention owing to their ability for sustained antibacterial activity. However, contact-killing antibacterial polystyrene (PS) has not been extensively studied because its chemically stable structure impedes chemical modification. In this study, we developed an antibacterial PS sheet with a contact-killing surface using PS synthesized from 2,2'-azobis-[2-(1,3-dimethyl-4,5-dihydro-1-imidazol-3-ium-2-yl)]propane triflate (ADIP) as a radical initiator with cationic moieties. The PS sheet synthesized with ADIP (ADIP-PS) exhibited antibacterial activity in contrast to PS synthesized with other azo radical initiators. Surface ζ-potential measurements revealed that only ADIP-PS had a cationic surface, which contributed to its contact-killing antibacterial activity. The ADIP-PS sheets also exhibited antibacterial activity after washing. In contrast, PS sheets containing silver, a typical leachable antibacterial agent, lost all antibacterial activity after the same washing treatment. The antibacterial ADIP-PS sheet demonstrated strong broad-spectrum activity against both Gram-positive and Gram-negative bacteria, including drug-resistant bacteria. Cytotoxicity tests using L929 cells showed that the ADIP-PS sheets were noncytotoxic. This contact-killing antibacterial PS synthesized with ADIP thus demonstrated good prospects as an easily producible antimicrobial material.
接触杀灭型抗菌材料因其具有持续抗菌活性的能力而备受关注。然而，接触杀灭型抗菌聚苯乙烯（PS）尚未得到广泛研究，因为其化学稳定结构阻碍了化学改性。在本研究中，我们使用由2,2'-偶氮二-[2-(1,3-二甲基-4,5-二氢-1-咪唑-3-鎓-2-基)]丙烷三氟甲磺酸盐（ADIP）作为带有阳离子部分的自由基引发剂合成的PS，开发了一种具有接触杀灭表面的抗菌PS片材。与用其他偶氮自由基引发剂合成的PS相比，用ADIP合成的PS片材（ADIP-PS）表现出抗菌活性。表面ζ电位测量表明，只有ADIP-PS具有阳离子表面，这有助于其接触杀灭抗菌活性。ADIP-PS片材在洗涤后也表现出抗菌活性。相比之下，含有典型可浸出抗菌剂银的PS片材在相同洗涤处理后失去了所有抗菌活性。抗菌ADIP-PS片材对革兰氏阳性菌和革兰氏阴性菌，包括耐药菌，都表现出强大的广谱活性。使用L929细胞进行的细胞毒性测试表明，ADIP-PS片材无细胞毒性。因此，这种用ADIP合成的接触杀灭型抗菌PS作为一种易于生产的抗菌材料显示出良好的前景。
