Gao Jing, Huddleston N Eric, White Evan M, Pant Jitendra, Handa Hitesh, Locklin Jason
Department of Chemistry, University of Georgia, Athens, Georgia 30602, United States.
Department of Chemistry and Biochemistry, University of North Georgia, Dahlonega, Georgia 30597, United States.
ACS Biomater Sci Eng. 2016 Jul 11;2(7):1169-1179. doi: 10.1021/acsbiomaterials.6b00221. Epub 2016 Jun 10.
In this work, we have investigated a quaternary ammonium compound that exhibits excellent antimicrobial activity and can be permanently grafted to substrates containing C-H bonds to form a durable polymeric film within 1 min. The compound consists of a biocidal component, dodecyl-alkylated quaternary ammonium, and a benzophenone moiety that, under mild UV irradiation, generates a densely cross-linked network and covalently attaches to a variety of substrates, including plastics, fabrics, and alkyl-modified glass surfaces. The surface attachment is 1 order of magnitude faster than that of previously reported benzophenone-associated cross-linkers, due to the electron-withdrawing effect of quaternary ammonium on the benzophenone chromophore. The modified surfaces are nonleaching and exhibit contact-killing and highly effective antimicrobial activity against (Gram-positive) and (Gram-negative) using cell count and live/dead staining methods. The charged ammonium group also promotes photoreaction efficiency with respect to network robustness, leading to a thin film that can sustain high shear forces and abrasion when compared to commercially available silane-based quaternary ammonium compounds. The biocidal activity is also retained after exposure to mechanical stress and abrasion.
在本研究中，我们研究了一种季铵化合物，该化合物具有出色的抗菌活性，能够永久接枝到含有碳氢键的底物上，并在1分钟内形成耐用的聚合物薄膜。该化合物由杀菌成分十二烷基烷基化季铵和二苯甲酮部分组成，在温和的紫外线照射下，二苯甲酮部分会形成密集交联网络，并与包括塑料、织物和烷基改性玻璃表面在内的多种底物共价连接。由于季铵对二苯甲酮发色团的吸电子作用，表面附着速度比先前报道的与二苯甲酮相关的交联剂快1个数量级。使用细胞计数和活/死染色方法，改性表面无浸出物，对革兰氏阳性菌和革兰氏阴性菌均表现出接触杀灭和高效抗菌活性。带电荷的铵基团还提高了网络稳健性方面的光反应效率，与市售的基于硅烷的季铵化合物相比，形成的薄膜能够承受高剪切力和磨损。在受到机械应力和磨损后，杀菌活性也得以保留。
