• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

通过深度测序和生物信息学分析鉴定鼻咽癌中的lncRNA/circRNA-miRNA-mRNA网络

Identification of a lncRNA/circRNA-miRNA-mRNA network in Nasopharyngeal Carcinoma by deep sequencing and bioinformatics analysis.

作者信息

Liu Shilei, Li Xiaoxiao, Xie Qingming, Zhang Sai, Liang Xujun, Li Shisheng, Zhang Pengfei

机构信息

NHC Key Laboratory of Cancer Proteomics, Department of Oncology, Xiangya Hospital, Central South University, Changsha, Hunan, P.R. China, 410008.

National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, Hunan, P.R. China, 410008.

出版信息

J Cancer. 2024 Feb 11;15(7):1916-1928. doi: 10.7150/jca.91546. eCollection 2024.

DOI:10.7150/jca.91546
PMID:38434987
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10905391/
Abstract

Accumulating evidence indicates that non-coding RNAs (ncRNA), including long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs), can function as competitive endogenous RNAs (ceRNAs) by binding to microRNAs (miRNAs) and regulating host gene expression at the transcriptional or post-transcriptional level. Dysregulation in ceRNA network regulation has been implicated in the occurrence and development of cancer. However, the lncRNA/circRNA-miRNA-mRNA regulatory network is still lacking in nasopharyngeal carcinoma (NPC). Differentially expressed genes (DEGs) were obtained from our previous sequencing data and Gene Expression Omnibus (GEO). Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes pathway (KEGG) were used to explore the biological functions of these common DEGs. Through a series of bioinformatic analyses, the lncRNA/circRNA-miRNA-mRNA network was established. In additional, the external data GSE102349 was used to test the prognostic value of the hub mRNAs through the Kaplan-Meier method. We successfully constructed a lncRNA/circRNA-miRNA-mRNA network in NPC, consisting of 16 lncRNAs, 6 miRNAs, 3 circRNAs and 10 mRNAs and found that three genes (TOP2A, ZWINT, TTK) were significantly associated with overall survival time (OS) in patients. The regulatory network revealed in this study may help comprehensively elucidate the ceRNA mechanisms driving NPC, and provide novel candidate biomarkers for evaluating the prognosis of NPC.

摘要

越来越多的证据表明，非编码RNA（ncRNA），包括长链非编码RNA（lncRNA）和环状RNA（circRNA），可以通过与微小RNA（miRNA）结合并在转录或转录后水平调节宿主基因表达，从而作为竞争性内源RNA（ceRNA）发挥作用。ceRNA网络调控失调与癌症的发生和发展有关。然而，鼻咽癌（NPC）中lncRNA/circRNA-miRNA-mRNA调控网络仍然缺乏。从我们之前的测序数据和基因表达综合数据库（GEO）中获得了差异表达基因（DEG）。利用基因本体论（GO）和京都基因与基因组百科全书通路（KEGG）来探索这些常见DEG的生物学功能。通过一系列生物信息学分析，建立了lncRNA/circRNA-miRNA-mRNA网络。此外，利用外部数据GSE102349通过Kaplan-Meier方法检验枢纽mRNA的预后价值。我们成功构建了鼻咽癌中的lncRNA/circRNA-miRNA-mRNA网络，该网络由16个lncRNA、6个miRNA、3个circRNA和10个mRNA组成，并发现三个基因（TOP2A、ZWINT、TTK）与患者的总生存时间（OS）显著相关。本研究揭示的调控网络可能有助于全面阐明驱动鼻咽癌的ceRNA机制，并为评估鼻咽癌的预后提供新的候选生物标志物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/edfd/10905391/f1b378c3e6f5/jcav15p1916g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/edfd/10905391/e3a234e12f54/jcav15p1916g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/edfd/10905391/36fa304d0ed0/jcav15p1916g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/edfd/10905391/f681dede7f92/jcav15p1916g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/edfd/10905391/d7a74f914361/jcav15p1916g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/edfd/10905391/b1bc48f5abde/jcav15p1916g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/edfd/10905391/853fd57b135f/jcav15p1916g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/edfd/10905391/f1b378c3e6f5/jcav15p1916g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/edfd/10905391/e3a234e12f54/jcav15p1916g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/edfd/10905391/36fa304d0ed0/jcav15p1916g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/edfd/10905391/f681dede7f92/jcav15p1916g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/edfd/10905391/d7a74f914361/jcav15p1916g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/edfd/10905391/b1bc48f5abde/jcav15p1916g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/edfd/10905391/853fd57b135f/jcav15p1916g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/edfd/10905391/f1b378c3e6f5/jcav15p1916g007.jpg

相似文献

1
Identification of a lncRNA/circRNA-miRNA-mRNA network in Nasopharyngeal Carcinoma by deep sequencing and bioinformatics analysis.通过深度测序和生物信息学分析鉴定鼻咽癌中的lncRNA/circRNA-miRNA-mRNA网络
J Cancer. 2024 Feb 11;15(7):1916-1928. doi: 10.7150/jca.91546. eCollection 2024.
2
Identification of a lncRNA/circRNA-miRNA-mRNA ceRNA Network in Alzheimer's Disease.鉴定阿尔茨海默病中的 lncRNA/circRNA-miRNA-mRNA ceRNA 网络。
J Integr Neurosci. 2023 Oct 17;22(6):136. doi: 10.31083/j.jin2206136.
3
Identification of Serum Exosome-Derived circRNA-miRNA-TF-mRNA Regulatory Network in Postmenopausal Osteoporosis Using Bioinformatics Analysis and Validation in Peripheral Blood-Derived Mononuclear Cells.基于生物信息学分析和外周血单核细胞验证鉴定绝经后骨质疏松症血清外泌体来源 circRNA-miRNA-TF-mRNA 调控网络
Front Endocrinol (Lausanne). 2022 Jun 9;13:899503. doi: 10.3389/fendo.2022.899503. eCollection 2022.
4
Excavating novel diagnostic and prognostic long non-coding RNAs (lncRNAs) for head and neck squamous cell carcinoma: an integrated bioinformatics analysis of competing endogenous RNAs (ceRNAs) and gene co-expression networks.挖掘新型诊断和预后长链非编码 RNA（lncRNA）对头颈鳞状细胞癌的作用：竞争性内源性 RNA（ceRNA）和基因共表达网络的综合生物信息学分析。
Bioengineered. 2021 Dec;12(2):12821-12838. doi: 10.1080/21655979.2021.2003925.
5
Integrated analysis of the lncRNA-miRNA-mRNA ceRNA network in nasopharyngeal carcinoma.鼻咽癌lncRNA-miRNA-mRNA ceRNA网络的综合分析
Transl Cancer Res. 2024 Aug 31;13(8):4372-4388. doi: 10.21037/tcr-24-263. Epub 2024 Aug 8.
6
Comprehensive analysis of the competing endogenous circRNA-lncRNA-miRNA-mRNA network and identification of a novel potential biomarker for hepatocellular carcinoma.全面分析竞争性内源性 circRNA-lncRNA-miRNA-mRNA 网络，并鉴定肝癌的新型潜在生物标志物。
Aging (Albany NY). 2021 May 28;13(12):15990-16008. doi: 10.18632/aging.203056.
7
Integrated analysis of lncRNA/circRNA-miRNA-mRNA in the proliferative phase of liver regeneration in mice with liver fibrosis.肝纤维化小鼠肝再生增殖期 lncRNA/circRNA-miRNA-mRNA 的整合分析。
BMC Genomics. 2023 Jul 24;24(1):417. doi: 10.1186/s12864-023-09478-z.
8
High-Throughput Sequencing and Exploration of the lncRNA-circRNA-miRNA-mRNA Network in Type 2 Diabetes Mellitus.高通量测序及 2 型糖尿病 lncRNA-circRNA-miRNA-mRNA 网络的探索。
Biomed Res Int. 2020 May 20;2020:8162524. doi: 10.1155/2020/8162524. eCollection 2020.
9
Construction and functional enrichment analysis of the competitive endogenous RNA regulatory network for nonarteritic anterior ischemic optic neuropathy based on high-throughput sequencing.基于高通量测序的非动脉炎性前部缺血性视神经病变竞争性内源性RNA调控网络的构建及功能富集分析
Funct Integr Genomics. 2022 Dec;22(6):1253-1267. doi: 10.1007/s10142-022-00914-z. Epub 2022 Nov 10.
10
Identification of circRNA-lncRNA-miRNA-mRNA Competitive Endogenous RNA Network as Novel Prognostic Markers for Acute Myeloid Leukemia.环状 RNA-lncRNA-miRNA-mRNA 竞争性内源性 RNA 网络作为急性髓系白血病新型预后标志物的鉴定。
Genes (Basel). 2020 Jul 31;11(8):868. doi: 10.3390/genes11080868.

引用本文的文献

1
RNA sequencing of the lncRNA regulome modulated by Withania somnifera in human neuroblastoma SK-N-SH cells.睡茄对人神经母细胞瘤SK-N-SH细胞lncRNA调控组的RNA测序
Mol Biol Rep. 2025 Sep 10;52(1):890. doi: 10.1007/s11033-025-10981-7.
2
Decoding potential lncRNA and disease associations through graph representation learning and gradient boosting with histogram.通过基于直方图的图表示学习和梯度提升来解码潜在的长链非编码RNA与疾病的关联。
Sci Rep. 2025 Aug 26;15(1):31407. doi: 10.1038/s41598-025-16177-0.
3
Functions and mechanisms of long non-coding RNA in esophageal squamous cell carcinoma (Review).

本文引用的文献

1
Increased Prevalence of EBV Infection in Nasopharyngeal Carcinoma Patients: A Six-Year Cross-Sectional Study.鼻咽癌患者中EB病毒感染患病率增加：一项为期六年的横断面研究。
Cancers (Basel). 2023 Jan 19;15(3):643. doi: 10.3390/cancers15030643.
2
Epigenetic control of chromosome-associated lncRNA genes essential for replication and stability.染色体相关长链非编码 RNA 基因的表观遗传控制对于复制和稳定性至关重要。
Nat Commun. 2022 Oct 22;13(1):6301. doi: 10.1038/s41467-022-34099-7.
3
A lncRNA signature associated with tumor immune heterogeneity predicts distant metastasis in locoregionally advanced nasopharyngeal carcinoma.
长链非编码RNA在食管鳞状细胞癌中的作用及机制（综述）
Oncol Lett. 2025 Jul 1;30(3):418. doi: 10.3892/ol.2025.15164. eCollection 2025 Sep.
4
Non-coding RNAs as key players in neurodegeneration and brain tumors: Insights into therapeutic strategies.非编码RNA作为神经退行性疾病和脑肿瘤的关键参与者：对治疗策略的见解
Iran J Basic Med Sci. 2025;28(8):962-985. doi: 10.22038/ijbms.2025.85350.18446.
5
Analysis of the Expression Patterns of Tumor Necrosis Factor Alpha Signaling Pathways and Regulatory MicroRNAs in Astrocytic Tumors.星形细胞瘤中肿瘤坏死因子α信号通路及调控性微小RNA的表达模式分析
Int J Mol Sci. 2025 Jun 19;26(12):5892. doi: 10.3390/ijms26125892.
6
LMFE: A Novel Method for Predicting Plant LncRNA Based on Multi-Feature Fusion and Ensemble Learning.LMFE：一种基于多特征融合与集成学习预测植物长链非编码RNA的新方法。
Genes (Basel). 2025 Mar 31;16(4):424. doi: 10.3390/genes16040424.
7
Current Research in Drug-Free Cancer Therapies.无药癌症治疗的当前研究
Bioengineering (Basel). 2025 Mar 26;12(4):341. doi: 10.3390/bioengineering12040341.
8
Integrative bioinformatics analysis of high-throughput sequencing and in vitro functional analysis leads to uncovering key hub genes in esophageal squamous cell carcinoma.高通量测序的整合生物信息学分析与体外功能分析有助于揭示食管鳞状细胞癌中的关键枢纽基因。
Hereditas. 2025 Mar 14;162(1):38. doi: 10.1186/s41065-025-00398-4.
9
The interplay of p16INK4a and non-coding RNAs: bridging cellular senescence, aging, and cancer.p16INK4a与非编码RNA的相互作用：连接细胞衰老、衰老和癌症
Biogerontology. 2025 Feb 5;26(2):50. doi: 10.1007/s10522-025-10194-2.
10
Bioinformatics and immunoinformatics approaches in the design of a multi-epitope vaccine targeting CTLA-4 for melanoma treatment.用于黑色素瘤治疗的靶向细胞毒性T淋巴细胞相关抗原4（CTLA-4）的多表位疫苗设计中的生物信息学和免疫信息学方法。
Mol Divers. 2025 Jan 28. doi: 10.1007/s11030-025-11108-7.
一个与肿瘤免疫异质性相关的 lncRNA 特征可预测局部晚期鼻咽癌的远处转移。
Nat Commun. 2022 May 30;13(1):2996. doi: 10.1038/s41467-022-30709-6.
4
The long and short non-coding RNAs modulating EZH2 signaling in cancer.长链和短链非编码 RNA 调节癌症中的 EZH2 信号通路。
J Hematol Oncol. 2022 Mar 2;15(1):18. doi: 10.1186/s13045-022-01235-1.
5
Human topoisomerases and their roles in genome stability and organization.人类拓扑异构酶及其在基因组稳定性和组织中的作用。
Nat Rev Mol Cell Biol. 2022 Jun;23(6):407-427. doi: 10.1038/s41580-022-00452-3. Epub 2022 Feb 28.
6
Specific expression and functions of circular RNAs.环状RNA的特异性表达及功能
Cell Death Differ. 2022 Mar;29(3):481-491. doi: 10.1038/s41418-022-00948-7. Epub 2022 Feb 15.
7
Diagnostic efficacy of circular RNAs as noninvasive, liquid biopsy biomarkers for early detection of gastric cancer.环状 RNA 作为非侵入性液体活检生物标志物用于早期胃癌检测的诊断效能。
Mol Cancer. 2022 Feb 9;21(1):42. doi: 10.1186/s12943-022-01527-7.
8
EBV infection-induced GPX4 promotes chemoresistance and tumor progression in nasopharyngeal carcinoma.EBV 感染诱导的 GPX4 促进鼻咽癌的化疗耐药和肿瘤进展。
Cell Death Differ. 2022 Aug;29(8):1513-1527. doi: 10.1038/s41418-022-00939-8. Epub 2022 Feb 1.
9
CircCCNB1 silencing acting as a miR-106b-5p sponge inhibited GPM6A expression to promote HCC progression by enhancing DYNC1I1 expression and activating the AKT/ERK signaling pathway.环状 CCNA1 沉默作为 miR-106b-5p 的海绵吸附物抑制 GPM6A 表达，通过增强 DYNC1I1 表达和激活 AKT/ERK 信号通路促进 HCC 进展。
Int J Biol Sci. 2022 Jan 1;18(2):637-651. doi: 10.7150/ijbs.66915. eCollection 2022.
10
Hypoxia-Induced ZWINT Mediates Pancreatic Cancer Proliferation by Interacting With p53/p21.缺氧诱导的ZWINT通过与p53/p21相互作用介导胰腺癌增殖。
Front Cell Dev Biol. 2021 Nov 24;9:682131. doi: 10.3389/fcell.2021.682131. eCollection 2021.