Dos Santos Quinton Marco, Avenant-Oldewage Annemariè
Department of Zoology, Faculty of Science, University of Johannesburg (Auckland Park Campus), Johannesburg, Gauteng, South Africa.
PeerJ. 2024 Feb 28;12:e17020. doi: 10.7717/peerj.17020. eCollection 2024.
The morphological characterisation of Diplozoidae spp. is highly reliant on the details of the sclerotised components of the hooks and clamps in the haptor. Only six species of (Diplozoinae) have been described from Africa, four of which have adequate morphological and even comparative ITS2 rDNA data available. However, the descriptions of (Thomas, 1957) and (Fischthal & Kuntz, 1963) lack essential taxonomic information, specifically the details for their haptoral sclerites. As such, all available material from museum collections for these two species were studied using light microscopy to supplement the original morphometric descriptions. The holotype and paratypes of were studied, but only voucher material for could be sourced. However, this voucher material for was deposited by the species authority and bore a striking resemblance to the illustrations and collection details from the original description. They were thus identified as the type series for the taxon, with a lectotype and paralectotype designated. Both and could be readily distinguished from other taxa based on the supplementary data generated here, supporting their distinctness. The haptoral sclerites of were most similar to those of Dos Santos & Avenant-Oldewage, 2016, also described from spp., while the sclerites of were most similar to Civáňová, Koyun & Koubková, 2013 and Al-Nasiri & Balbuena, 2016. Additionally, a voucher of collected from the alestid type host of was reidentified as the latter species here. This greatly simplified the known host specificity for spp. in Africa, with now exclusively reported from Cypriniformes (Cyprinidae and Danionidae), and restricted to Characiformes (Alestidae). The occurrence of all diplozoids from non-cyprinoid hosts was also investigated and several records of diplozoids occurring on non-cyprinoid hosts were collated and scrutinised. Excluding the two instances of diplozoids described and exclusively occurring on Characiformes fishes ( and (Sterba, 1957)), most other non-cyprinoid collections appear sporadic and unsubstantiated, but warrant further investigation supported by diligent taxonomic data. Even though the morphometric descriptions of both and were fully reported on here, additional material will be needed to study their genetic profiles and phylogeny.
双殖吸虫科物种的形态特征高度依赖于钩介幼虫中钩和夹的硬化部分的细节。非洲仅描述了6种（双殖吸虫亚科），其中4种有足够的形态学数据，甚至有比较ITS2 rDNA数据。然而，托马斯（1957年）和菲施塔尔与昆茨（1963年）的描述缺乏关键的分类信息，特别是它们的钩介幼虫硬体部分的细节。因此，利用光学显微镜对博物馆收藏的这两个物种的所有可用材料进行了研究，以补充原始形态测量描述。研究了 的正模标本和副模标本，但仅找到了 的凭证标本。然而，这个 的凭证标本是由物种权威机构保存的，与原始描述中的插图和采集细节惊人地相似。因此，它们被确定为该分类单元的模式系列，并指定了一个选模和副选模。根据此处生成的补充数据， 和 都可以很容易地与其他分类单元区分开来，这支持了它们的独特性。 的钩介幼虫硬体部分与2016年多斯桑托斯和阿韦南特 - 奥德维奇也从 物种中描述的最为相似，而 的硬体部分与2013年奇瓦诺娃、科云与库布科娃以及2016年阿尔 - 纳西里与巴尔布埃纳的最为相似。此外，从 的艾氏鱼属型宿主采集的一个 的凭证标本在此被重新鉴定为后一个物种。这大大简化了非洲 物种已知的宿主特异性，现在 仅从鲤形目（鲤科和丹亚科）中报道，而 仅限于脂鲤目（艾氏鱼科）。还调查了所有来自非鲤形目宿主的双殖吸虫的出现情况，并整理和审查了一些关于双殖吸虫出现在非鲤形目宿主上的记录。除了描述的并仅出现在脂鲤目鱼类上的两种双殖吸虫（ 和 （斯特尔巴，1957年））外，大多数其他非鲤形目宿主的采集似乎是零星的且未经证实，但需要在详细的分类数据支持下进行进一步调查。尽管此处充分报道了 和 的形态测量描述，但仍需要更多材料来研究它们的基因图谱和系统发育。
