Center for Endocrine Metabolism and Immune Diseases, Beijing Luhe Hospital, Capital Medical University, Beijing, China.
Clin Exp Rheumatol. 2024 Jul;42(7):1442-1450. doi: 10.55563/clinexprheumatol/7pcgv7. Epub 2024 Mar 3.
The aberrant expression of omentin-1 had been reported in type 2 diabetes and cardiovascular disease. Here, we investigated the expression and role of omentin-1 in rheumatoid arthritis (RA).
The expression of omentin-1 in RA and in the normal population was detected by ELISA and immunohistochemistry, and collagen-induced arthritis (CIA) mice were used to detect the role of omentin-1 in RA.
We found that the expression of omentin-1 was elevated in serum of RA patients compared with healthy controls (p=0.004), and in the RA disease activity group compared with the disease remission group (p<0.001). In addition, the level of omentin-1 in RA patients was positively correlated with CRP (r=0.427, p=0.002), ESR (r=0.454, p<0.001) and DAS28 (r=0.496, p<0.001; r=0.661, p<0.001, respectively). Multivariable analysis showed that omentin-1 alone was associated with disease activity state (OR=1.018, p=0.004). Immunohistochemical results showed that omentin-1 was increased in the synovium of RA and CIA mice. Omentin-1 injection resulted in an earlier onset of arthritis, an aggravated arthritic progression, more severe synovial hyperplasia and bone erosion in CIA mice. Moreover, omentin-1 treatment markedly enhanced IL-6, TNF-α, MMP-3, MMP-13 and RANKL in the joint tissue of CIA mice.
Our results suggested that omentin-1 was up-regulated in RA and can exacerbate synovitis and joint destruction which may provide new insight into the pathogenesis of RA.
已有研究报道网膜素-1 在 2 型糖尿病和心血管疾病中表达异常。本研究旨在探讨网膜素-1 在类风湿关节炎（RA）中的表达和作用。
采用 ELISA 和免疫组化法检测 RA 患者和正常人群网膜素-1 的表达，并使用胶原诱导性关节炎（CIA）小鼠检测网膜素-1 在 RA 中的作用。
我们发现，与健康对照组相比，RA 患者血清中网膜素-1 的表达升高（p=0.004），且在疾病活动组高于疾病缓解组（p<0.001）。此外，RA 患者网膜素-1 水平与 CRP（r=0.427，p=0.002）、ESR（r=0.454，p<0.001）和 DAS28（r=0.496，p<0.001；r=0.661，p<0.001）呈正相关。多变量分析显示，网膜素-1 与疾病活动状态独立相关（OR=1.018，p=0.004）。免疫组化结果显示，RA 患者滑膜和 CIA 小鼠滑膜中网膜素-1 增加。给予网膜素-1 注射可使 CIA 小鼠关节炎更早发作，关节炎进展加重，滑膜增生和骨侵蚀更严重。此外，网膜素-1 治疗可显著增强 CIA 小鼠关节组织中 IL-6、TNF-α、MMP-3、MMP-13 和 RANKL 的表达。
我们的研究结果表明，RA 中网膜素-1 表达上调，并可加重滑膜炎和关节破坏，这可能为 RA 的发病机制提供新的见解。
