Key Laboratory of Specialty Agri-product Quality and Hazard Controlling Technology of Zhejiang Province, College of Life Sciences, China Jiliang University, Hangzhou, People's Republic of China.
J Sci Food Agric. 2024 Aug 15;104(10):6008-6017. doi: 10.1002/jsfa.13429. Epub 2024 Mar 18.
The alteration of chromatin accessibility plays an important role in plant responses to abiotic stress. Carbon-based nanomaterials (CBNMs) have attracted increasing interest in agriculture due to their potential impact on crop productivity, showcasing effects on plant biological processes at transcriptional levels; however, their impact on chromatin accessibility remains unknown.
This study found that fullerenol can penetrate the seed coat of pea to mitigate the reduction of seed germination caused by osmotic stress. RNA sequencing (RNA-seq) revealed that the application of fullerenol caused the high expression of genes related to oxidoreduction to return to a normal level. Assay for transposase accessible chromatin sequencing (ATAC-seq) confirmed that fullerenol application reduced the overall levels of chromatin accessibility of numerous genes, including those related to environmental signaling, transcriptional regulation, and metabolism.
This study suggests that fullerenol alleviates osmotic stress on various fronts, encompassing antioxidant, transcriptional, and epigenetic levels. This advances knowledge of the working mechanism of this nanomaterial within plant cells. © 2024 Society of Chemical Industry.
染色质可及性的改变在植物应对非生物胁迫中起着重要作用。由于碳基纳米材料（CBNMs）可能对作物生产力产生影响，在转录水平上展示了对植物生物过程的影响，因此在农业中引起了越来越多的关注；然而，其对染色质可及性的影响尚不清楚。
本研究发现，富勒醇可以穿透豌豆种子的种皮，减轻渗透胁迫导致的种子发芽率降低。RNA 测序（RNA-seq）显示，富勒醇的应用使与氧化还原相关的基因的高表达恢复到正常水平。转座酶可及染色质测序（ATAC-seq）分析证实，富勒醇的应用降低了许多基因的整体染色质可及性水平，包括与环境信号、转录调控和代谢相关的基因。
本研究表明，富勒醇从抗氧化、转录和表观遗传等多个层面缓解了渗透胁迫。这加深了我们对这种纳米材料在植物细胞内工作机制的认识。© 2024 化学工业协会。
