Jiangsu Engineering Research Center of Cardiovascular Drugs Targeting Endothelial Cells, College of Health Sciences, School of Life Sciences, Jiangsu Normal University, Xuzhou 221116, Jiangsu, China.
School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin 150090, Heilongjiang, China.
Bioorg Chem. 2024 Apr;145:107232. doi: 10.1016/j.bioorg.2024.107232. Epub 2024 Feb 21.
This study investigated the stability of milk fat globule membrane (MFGM) protein under simulated gastrointestinal conditions using an in vitro enzymatic digestion method. The optimal hydrolysis conditions were determined by monitoring the changes in particle size and zeta-potential of MFGM protein hydrolysates over time. Furthermore, the distribution of small molecular weight peptides with antioxidant activity was explored through DEAE-52 combined with in vitro cell experiments. Two novel antioxidant peptides (TGIIT and IITQ) were identified based on molecular docking technology and evaluated their potential scavenging activity against 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-Azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) radicals. TGIIT and IITQ also demonstrated remarkable abilities in promoting mitochondrial biogenesis and activating Keap1/Nrf2 signaling pathway, which can effectively counteract skeletal muscle dysfunction induced by oxidative stress. Thus, MFGM-derived antioxidant peptides have the potential to be employed in food to regulate muscle protein metabolism and alleviate sarcopenia.
本研究采用体外酶解方法，研究了模拟胃肠道条件下乳脂肪球膜（MFGM）蛋白的稳定性。通过监测 MFGM 蛋白水解产物粒径和 zeta 电位随时间的变化，确定了最佳水解条件。此外，还通过 DEAE-52 结合体外细胞实验，探讨了具有抗氧化活性的小分子肽的分布。基于分子对接技术，鉴定了两种新型抗氧化肽（TGIIT 和 IITQ），并评估了它们对 1,1-二苯基-2-苦基肼基（DPPH）和 2,2'-联氮-双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）自由基的潜在清除活性。TGIIT 和 IITQ 还表现出促进线粒体生物发生和激活 Keap1/Nrf2 信号通路的显著能力，可有效抵抗氧化应激引起的骨骼肌功能障碍。因此，MFGM 衍生的抗氧化肽有可能被应用于食品中，以调节肌肉蛋白质代谢并缓解肌肉减少症。
