Jiangsu Engineering Research Center of Cardiovascular Drugs Targeting Endothelial Cells, College of Health Sciences, School of Life Sciences, Jiangsu Normal University, Xuzhou 221116, Jiangsu, China.
Jiangsu Engineering Research Center of Cardiovascular Drugs Targeting Endothelial Cells, College of Health Sciences, School of Life Sciences, Jiangsu Normal University, Xuzhou 221116, Jiangsu, China; School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin 150090, Heilongjiang, China.
Food Chem. 2023 Mar 1;403:134473. doi: 10.1016/j.foodchem.2022.134473. Epub 2022 Oct 3.
Milk fat globule membrane (MFGM) protein is a complex milk protein system with antioxidant property, which can alleviate skeletal muscle dysfunction caused by oxidative stress. In this study, peptide products of MFGM protein obtained through in vitro digestion were isolated and purified, and the composition and antioxidant activities of MFGM peptides (MFGMP) were identified and assessed using LC-MS/MS combined with molecular docking and in vitro approach. Three novel antioxidant peptides TGIIT, YAR and YYK were identified from MFGMPF1, among which TGIIT and YAR exhibited excellent antioxidant effects and protected dexamethasone (Dex)-induced L6 cells by enhancing mitochondrial function and biogenesis involving modulating Sirt-1/PGC-1α signaling pathway. Furthermore, YAR and TGIIT also significantly decreased expression of pro-apoptotic factors such as cyt-c, cleaved caspase-3 and caspase-9. Therefore, YAR and TGIIT, two novel antioxidant peptides, are expected to be utilized in functional food or medicine, providing an emerging role of MFGMP in maintaining anti-oxidant/oxidant status.
乳脂肪球膜（MFGM）蛋白是一种具有抗氧化特性的复杂乳蛋白系统，可减轻由氧化应激引起的骨骼肌功能障碍。在这项研究中，通过体外消化获得 MFGM 蛋白的肽产物被分离和纯化，并使用 LC-MS/MS 结合分子对接和体外方法鉴定和评估 MFGM 肽（MFGMP）的组成和抗氧化活性。从 MFGMPF1 中鉴定出三种新型抗氧化肽 TGIIT、YAR 和 YYK，其中 TGIIT 和 YAR 表现出优异的抗氧化作用，并通过增强涉及调节 Sirt-1/PGC-1α 信号通路的线粒体功能和生物发生来保护地塞米松（Dex）诱导的 L6 细胞。此外，YAR 和 TGIIT 还显著降低了细胞色素 c、裂解的 caspase-3 和 caspase-9 等促凋亡因子的表达。因此，两种新型抗氧化肽 YAR 和 TGIIT 有望在功能性食品或药物中得到应用，为 MFGMP 在维持抗氧化/氧化状态方面提供了新的作用。
