College of Horticulture, Fujian Agriculture and Forestry University, 350002, Fuzhou, China.
Anxi College of Tea Science, Fujian Agriculture and Forestry University, 350028, Quanzhou, China.
BMC Genomics. 2024 Mar 4;25(1):238. doi: 10.1186/s12864-024-09972-y.
The caffeoyl-CoA-O methyltransferase (CCoAOMT) family plays a crucial role in the oxidative methylation of phenolic substances and is involved in various plant processes, including growth, development, and stress response. However, there is a limited understanding of the interactions among CCoAOMT protein members in tea plants.
In this study, we identified 10 members of the CsCCoAOMT family in the genome of Camellia sinensis (cultivar 'HuangDan'), characterized by conserved gene structures and motifs. These CsCCoAOMT members were located on six different chromosomes (1, 2, 3, 4, 6, and 14). Based on phylogenetic analysis, CsCCoAOMT can be divided into two groups: I and II. Notably, the CsCCoAOMT members of group Ia are likely to be candidate genes involved in lignin biosynthesis. Moreover, through the yeast two-hybrid (Y2H) assay, we established protein interaction networks for the CsCCoAOMT family, revealing 9 pairs of members with interaction relationships.
We identified the CCoAOMT gene family in Camellia sinensis and conducted a comprehensive analysis of their classifications, phylogenetic and synteny relationships, gene structures, protein interactions, tissue-specific expression patterns, and responses to various stresses. Our findings shed light on the evolution and composition of CsCCoAOMT. Notably, the observed interaction among CCoAOMT proteins suggests the potential formation of the O-methyltransferase (OMT) complex during the methylation modification process, expanding our understanding of the functional roles of this gene family in diverse biological processes.
咖啡酰辅酶 A-O-甲基转移酶（CCoAOMT）家族在酚类物质的氧化甲基化中起着至关重要的作用，参与各种植物过程，包括生长、发育和应激反应。然而，对茶树中 CCoAOMT 蛋白成员之间的相互作用知之甚少。
本研究在茶树基因组中鉴定了 10 个 CsCCoAOMT 家族成员，其基因结构和基序保守。这些 CsCCoAOMT 成员位于 6 条不同的染色体（1、2、3、4、6 和 14）上。基于系统发育分析，CsCCoAOMT 可分为两组：I 和 II。值得注意的是，组 Ia 的 CsCCoAOMT 成员可能是参与木质素生物合成的候选基因。此外，通过酵母双杂交（Y2H）试验，我们建立了 CsCCoAOMT 家族的蛋白质相互作用网络，揭示了 9 对具有相互作用关系的成员。
我们鉴定了茶树中的 CCoAOMT 基因家族，并对其分类、系统发育和共线性关系、基因结构、蛋白质相互作用、组织特异性表达模式以及对各种胁迫的响应进行了全面分析。我们的研究结果揭示了 CsCCoAOMT 的进化和组成。值得注意的是，观察到的 CCoAOMT 蛋白之间的相互作用表明，在甲基化修饰过程中可能形成 O-甲基转移酶（OMT）复合物，这扩展了我们对该基因家族在各种生物过程中功能作用的理解。
