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L.基因家族的全基因组鉴定与表达特征分析

Genome-Wide Identification and Expression Characterization of the Gene Family of L.

作者信息

Wu Di, Fu Wenting, Wang Nanyi, Ye Yong, He Jianwen, Wu Kangyun

机构信息

Research Institute of Pepper, Guizhou Academy of Agricultural Science, Guiyang 550025, China.

Key Laboratory of Crop Genetic Resources and Germplasm Innovation in Karst Mountain Areas, Ministry of Agriculture and Rural Affairs, Guiyang 550025, China.

出版信息

Plants (Basel). 2024 Jul 26;13(15):2070. doi: 10.3390/plants13152070.

Abstract

As a crucial member of the gene family involved in the biosynthesis of strigolactones, plays an important regulatory role in plant branching and root development, which is essential for field management and yield increase in peppers ( L.). To comprehensively understand the characteristics of the pepper gene family, we identified three genes. By analyzing their physicochemical properties, phylogenetic relationships, gene structures, promoters, and expression patterns in different tissues, the characteristics of the gene family were revealed. The research results showed that these three genes are located in three different chromosomes. Evolutionary analysis divided the members of CaD27 into three groups, and gene collinearity analysis did not find any duplicates, indicating the diversity and non-redundancy of the gene family members. In addition, we identified and classified -elements in the promoter regions of genes, with a relatively high proportion related to light and plant hormone responses. Expression pattern analysis showed that is expressed in leaves, while is expressed in roots, indicating tissue specificity. Furthermore, protein interaction predictions revealed an interaction between D27.2 and CCD7. This study provided important insights into the function and regulatory mechanisms of the gene family and the role of strigolactones in plant growth and development.

摘要

作为参与独脚金内酯生物合成的基因家族的关键成员,在植物分枝和根系发育中发挥重要调控作用,这对辣椒( )的田间管理和增产至关重要。为全面了解辣椒 基因家族的特征,我们鉴定了三个 基因。通过分析它们的理化性质、系统发育关系、基因结构、启动子以及在不同组织中的表达模式,揭示了 基因家族的特征。研究结果表明,这三个 基因位于三条不同的染色体上。进化分析将CaD27成员分为三组,基因共线性分析未发现任何重复,表明 基因家族成员的多样性和非冗余性。此外,我们鉴定并分类了 基因启动子区域中的 -元件,其中与光和植物激素反应相关的比例相对较高。表达模式分析表明, 在叶片中表达,而 在根中表达,表明具有组织特异性。此外,蛋白质相互作用预测揭示了D27.2与CCD7之间的相互作用。本研究为 基因家族的功能和调控机制以及独脚金内酯在植物生长发育中的作用提供了重要见解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/07e9/11314413/d098e39e6d55/plants-13-02070-g001.jpg

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