外周血RNA中的N-甲基腺苷水平：一种潜在的结直肠癌诊断生物标志物。

N-methyladenosine levels in peripheral blood RNA: a potential diagnostic biomarker for colorectal cancer.

作者信息

Zhang Chunying, Chen Jiadi, Ren Jingyi, Li Xiaoyu, Zhang Yaqin, Huang Bihan, Xu Yihan, Dong Luyan, Cao Yingping

机构信息

Department of Clinical Laboratory, Fujian Medical University Union Hospital, Fuzhou, China.

出版信息

Cancer Cell Int. 2024 Mar 5;24(1):96. doi: 10.1186/s12935-024-03289-2.

DOI:10.1186/s12935-024-03289-2

PMID:38439072

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10913687/

Abstract

BACKGROUND

N-methyladenosine (mA) is dysregulated in various cancers, including colorectal cancer (CRC). Herein, we assess the diagnostic potential of peripheral blood (PB) mA levels in CRC.

METHODS

We collected PB from healthy controls (HCs) and patients with CRC, analyzed PB RNA mA levels and the expression of mA-related demethylase genes FTO and ALKBH5, cocultured CRC cells with PB mononuclear cells (PBMCs), and constructed an MC38 cancer model.

RESULTS

PB RNA mA levels were higher in the CRC than that in HCs. The area under the curve (AUC) of mA levels (0.886) in the CRC was significantly larger compared with carbohydrate antigen 199 (CA199; 0.666) and carcinoembryonic antigen (CEA; 0.834). The combination of CEA and CA199 with PB RNA mA led to an increase in the AUC (0.935). Compared with HCs, the expression of FTO and ALKBH5 was decreased in the CRC. After coculturing with CRC cells, the PBMCs RNA mA were significantly increased, whereas the expression of FTO and ALKBH5 decreased. Furthermore, mA RNA levels in the PB of MC38 cancer models were upregulated, whereas the expression of FTO and ALKBH5 decreased.

CONCLUSIONS

PB RNA mA levels are a potential diagnostic biomarker for patients with CRC.

摘要

背景

N - 甲基腺苷（mA）在包括结直肠癌（CRC）在内的多种癌症中存在失调。在此，我们评估外周血（PB）中mA水平在CRC中的诊断潜力。

方法

我们收集了健康对照者（HCs）和CRC患者的PB，分析PB RNA mA水平以及mA相关去甲基化酶基因FTO和ALKBH5的表达，将CRC细胞与PB单核细胞（PBMCs）共培养，并构建了MC38癌症模型。

结果

CRC患者PB RNA mA水平高于HCs。CRC中mA水平的曲线下面积（AUC）（0.886）显著大于糖类抗原199（CA199；0.666）和癌胚抗原（CEA；0.834）。CEA和CA199与PB RNA mA联合使用导致AUC增加（0.935）。与HCs相比，CRC中FTO和ALKBH5的表达降低。与CRC细胞共培养后，PBMCs的RNA mA显著增加，而FTO和ALKBH5的表达降低。此外，MC38癌症模型PB中的mA RNA水平上调，而FTO和ALKBH5的表达降低。