Vered M, Dearing R, Janoff A
Am Rev Respir Dis. 1985 Jan;131(1):131-3. doi: 10.1164/arrd.1985.131.1.131.
A neutrophil elastase-inhibitor isolated from lysed pneumococcal cells, as well as trypsin-digest peptides derived from this factor, were tested for their ability to suppress acute lung injury in mice treated with human neutrophil granule extracts. Injury was assessed by measuring pulmonary sequestration of circulating 125I-labeled albumin, lung water, and lung hemoglobin. Both the native inhibitor and the tryptic-peptides gave good protection when preincubated with granule extract for brief periods before intrapulmonary instillation. Lesser, but still significant, protection was observed in the absence of preincubation. Protection was not simply due to addition of exogenous proteins to the granule extract because substitution of goat immunoglobulin for pneumococcal fraction was ineffective. These results suggest that pneumococcal elastase-inhibitors, recently described by us, may play a role in minimizing lung injury during pneumococcal pneumonia.
从裂解的肺炎球菌细胞中分离出的一种中性粒细胞弹性蛋白酶抑制剂，以及源自该因子的胰蛋白酶消化肽，被测试了它们抑制用人中性粒细胞颗粒提取物处理的小鼠急性肺损伤的能力。通过测量循环中125I标记白蛋白的肺内潴留、肺含水量和肺血红蛋白来评估损伤。在肺内滴注前，将天然抑制剂和胰蛋白酶肽与颗粒提取物短暂预孵育时，两者都提供了良好的保护。在没有预孵育的情况下，观察到的保护作用较小，但仍然显著。保护作用并非仅仅由于向颗粒提取物中添加了外源蛋白质，因为用山羊免疫球蛋白替代肺炎球菌组分是无效的。这些结果表明，我们最近描述的肺炎球菌弹性蛋白酶抑制剂可能在最小化肺炎球菌肺炎期间的肺损伤中发挥作用。
