McDonald R J, Bruckner L V, Repine J E
Department of Pediatrics, University of California, Davis.
Am Rev Respir Dis. 1989 Dec;140(6):1825-7. doi: 10.1164/ajrccm/140.6.1825.
To assess the contribution of neutrophil elastase to neutrophil-mediated acute edematous lung injury, isolated rat lungs were perfused with human neutrophil cytoplasts that produce O2 metabolites normally but contain little of the neutrophil elastase usually found in neutrophils. We found that addition of neutrophil cytoplasts and phorbol myristate acetate (PMA) caused less edematous injury in isolated lungs than addition of neutrophils and PMA. However, addition of neutrophil elastase along with neutrophil cytoplasts and PMA increased the amounts of lung injury found in lungs perfused with neutrophil cytoplasts and PMA to levels that equaled the amount of lung injury found after addition of normal neutrophils and PMA. By comparison, addition of purified neutrophil elastase, PMA alone, or human neutrophils alone did not cause lung injury. The results indicate that neutrophil elastase is a necessary component for the maximal development of acute edematous injury in isolated lungs perfused with neutrophils and PMA.
为评估中性粒细胞弹性蛋白酶在中性粒细胞介导的急性肺水肿性肺损伤中的作用，用通常能产生O2代谢产物但几乎不含中性粒细胞中常见的中性粒细胞弹性蛋白酶的人中性粒细胞胞质体灌注离体大鼠肺。我们发现，与添加中性粒细胞和佛波酯肉豆蔻酸酯（PMA）相比，添加中性粒细胞胞质体和PMA在离体肺中引起的水肿性损伤更小。然而，将中性粒细胞弹性蛋白酶与中性粒细胞胞质体和PMA一起添加，会使灌注中性粒细胞胞质体和PMA的肺中发现的肺损伤量增加到与添加正常中性粒细胞和PMA后发现的肺损伤量相当的水平。相比之下，单独添加纯化的中性粒细胞弹性蛋白酶、单独的PMA或单独的人中性粒细胞不会引起肺损伤。结果表明，中性粒细胞弹性蛋白酶是在用中性粒细胞和PMA灌注的离体肺中急性水肿性损伤最大程度发展的必要成分。
