Loscutoff S M, Cannon W C, Buschbom R L, Busch R H, Killand B W
Environ Res. 1985 Feb;36(1):170-80. doi: 10.1016/0013-9351(85)90015-5.
Three weeks following intratracheal instillations of elastase dissolved in saline, or saline alone, guinea pigs and rats were exposed for 5 or 20 days, 6 hr/day, 5 days/week to filtered room air, 1 mg/m3 ammonium sulfate [NH4)2SO4) or 1 mg/m3 ammonium nitrate (NH4NO3) aerosols. Pulmonary function evaluations conducted in guinea pigs showed no detrimental effects of (NH4)2SO4 or NH4NO3 exposure and very little effect of elastase treatment. Lung function changes in elastase-treated rats were consistent with a condition of experimentally induced pulmonary emphysema. Rats exposed to NH4NO3 aerosols showed no consistent exposure-related changes. Compared with air-exposed animals, rats exposed to (NH4)2SO4 aerosols had increased values of residual volume and functional residual capacity and decreased slope of single-breath N2 washout curves. We conclude that elastase treatment had no significant effect on lung function changes resulting from inhalation of (NH4)2SO4 aerosols. Lung function was more affected by (NH4)2SO4 exposure than by NH4NO3 exposure, and lung function changes were more pronounced in rats than in guinea pigs.
在气管内注入溶解于生理盐水中的弹性蛋白酶或仅注入生理盐水三周后，将豚鼠和大鼠每周5天、每天6小时暴露于过滤后的室内空气、1毫克/立方米硫酸铵[(NH₄)₂SO₄]或1毫克/立方米硝酸铵(NH₄NO₃)气溶胶中，持续5天或20天。对豚鼠进行的肺功能评估显示，暴露于(NH₄)₂SO₄或NH₄NO₃没有有害影响，弹性蛋白酶处理的影响也很小。弹性蛋白酶处理的大鼠的肺功能变化与实验性诱发的肺气肿状况一致。暴露于NH₄NO₃气溶胶的大鼠没有显示出与暴露相关的一致变化。与暴露于空气的动物相比，暴露于(NH₄)₂SO₄气溶胶的大鼠的残气量和功能残气量增加，单次呼吸氮气洗脱曲线的斜率降低。我们得出结论，弹性蛋白酶处理对吸入(NH₄)₂SO₄气溶胶引起的肺功能变化没有显著影响。与NH₄NO₃暴露相比，(NH₄)₂SO₄暴露对肺功能的影响更大，并且大鼠的肺功能变化比豚鼠更明显。
