Schapkaitz Elise, Pillay Dashini
Department of Molecular Medicine and Haematology, Charlotte Maxeke Johannesburg Academic Hospital, National Health Laboratory System Complex and University of Witwatersrand, South Africa.
Department of Haematology, National Health Laboratory Services and University of KwaZulu- Natal, South Africa.
Afr J Lab Med. 2015 Aug 31;4(1):208. doi: 10.4102/ajlm.v4i1.208. eCollection 2015.
Referral of samples for the work-up of haematological disorders from remote laboratories can result in a delay in analysis.
The stability of the full blood count (FBC), differential count (DIFF), reticulocyte and peripheral blood smear (PBS) morphology during extended storage was evaluated.
Forty blood samples stored in ethylenediaminetetraacetic acid (EDTA) were analysed on an ADVIA® 120 haematology analyser. The samples (25% abnormal; 75% normal) were stored at room temperature (RT) and at 4 °C - 8 °C. Analysis of samples stored at RT was performed every 12 hours for two days. Analysis of samples stored at 4 °C - 8 °C was performed at 12 hours and subsequently every 24 hours for seven days.
FBC parameters (red cell count, haemoglobin) and DIFF parameters (percentages of basophils, lymphocytes and monocytes) were stable for at least 48 hours when stored at RT. Platelets were only stable for 12 hours and the white cell count was stable for 36 hours when stored at RT. Storing samples at 4 °C - 8 °C significantly increased the stability of most parameters, in particular, mean cell volume and percentage of reticulocytes. However, DIFF parameters were associated with lower stability at 4 °C - 8 °C. PBS morphology was compromised prior to 12 hours whether stored at RT or at 4 °C - 8 °C.
This study provides evidence that blood samples stored in EDTA at 4 °C - 8 °C for seven days are suitable for testing on the ADVIA® 120 analyser for the FBC and percentage of reticulocyte parameters. However, storage at 4 °C - 8 °C is not a solution for samples referred for DIFF and PBS morphology review.
将用于血液系统疾病检查的样本从偏远实验室送检可能会导致分析延迟。
评估全血细胞计数（FBC）、分类计数（DIFF）、网织红细胞和外周血涂片（PBS）形态在延长储存期间的稳定性。
在ADVIA® 120血液分析仪上对40份储存在乙二胺四乙酸（EDTA）中的血液样本进行分析。这些样本（25%异常；75%正常）分别储存在室温（RT）以及4°C - 8°C环境下。储存在室温的样本在两天内每12小时进行一次分析。储存在4°C - 8°C的样本在12小时时进行一次分析，随后在七天内每24小时进行一次分析。
储存在室温时，FBC参数（红细胞计数、血红蛋白）和DIFF参数（嗜碱性粒细胞、淋巴细胞和单核细胞百分比）至少在48小时内保持稳定。血小板在室温储存时仅稳定12小时，白细胞计数在室温储存时稳定36小时。将样本储存在4°C - 8°C显著提高了大多数参数的稳定性，特别是平均红细胞体积和网织红细胞百分比。然而，DIFF参数在4°C - 8°C时稳定性较低。无论储存在室温还是4°C - 8°C，PBS形态在12小时之前就会受到影响。
本研究表明，储存在4°C - 8°C的EDTA血液样本在七天内适用于在ADVIA® 120分析仪上进行FBC和网织红细胞百分比参数检测。然而，对于送检进行DIFF和PBS形态检查的样本，4°C - 8°C储存并非解决方案。
