Liu Jianbai, Liu Huan, Yi Jiequn, Mao Yongkai, Li Jihu, Sun Donglei, An Yuxing, Wu Han
Guangdong Engineering Research Center for Pesticide and Fertilizer, Institute of Bioengineering, Guangdong Academy of Sciences, Guangzhou, China.
College of Horticulture and Plant Protection, Henan University of Science and Technology, Luoyang, China.
Front Physiol. 2021 Mar 5;12:636353. doi: 10.3389/fphys.2021.636353. eCollection 2021.
Insect chemoreception involves many families of genes, including odourant/pheromone binding proteins (), chemosensory proteins (), odourant receptors (), ionotropic receptors (), and sensory neuron membrane proteins (), which play irreplaceable roles in mediating insect behaviors such as host location, foraging, mating, oviposition, and avoidance of danger. However, little is known about the molecular mechanism of olfactory reception in , which is a major pest of sugarcane. A set of 72 candidate chemosensory genes, including 31 , 15 , 11 , 13 , and two , were identified in four transcriptomes from different tissues and genders of . Phylogenetic analysis was conducted on gene families and paralogs from other model insect species. Quantitative real-time PCR (qRT-PCR) showed that most of these chemosensory genes exhibited antennae-biased expression, but some had high expression in bodies. Most of the identified chemosensory genes were likely involved in chemoreception. This study provides a molecular foundation for the function of chemosensory proteins, and an opportunity for understanding how behaviors are mediated via chemical cues. This research might facilitate the discovery of novel strategies for pest management in agricultural ecosystems.
昆虫化学感受涉及许多基因家族，包括气味/信息素结合蛋白、化学感受蛋白、气味受体、离子型受体和感觉神经元膜蛋白，它们在介导昆虫行为（如宿主定位、觅食、交配、产卵和躲避危险）中发挥着不可替代的作用。然而，对于甘蔗的主要害虫[具体害虫名称未给出]嗅觉接收的分子机制知之甚少。从[具体昆虫名称未给出]不同组织和性别的四个转录组中鉴定出一组72个候选化学感受基因，包括31个[具体基因类型未给出1]、15个[具体基因类型未给出2]、11个[具体基因类型未给出3]、13个[具体基因类型未给出4]和两个[具体基因类型未给出5]。对来自其他模式昆虫物种的基因家族和旁系同源基因进行了系统发育分析。实时定量PCR（qRT-PCR）表明，这些化学感受基因中的大多数表现出触角偏向性表达，但有些在体内有高表达。大多数鉴定出的化学感受基因可能参与化学感受。本研究为化学感受蛋白的功能提供了分子基础，并为理解[具体昆虫名称未给出]行为如何通过化学信号介导提供了契机。这项研究可能有助于发现农业生态系统中害虫管理的新策略。
