Department of Clinical Medicine & Surgery, University of Naples 'Federico II', 80131, Naples, Italy.
CEINGE, Biotecnologie Avanzate S.c.a.r.l., 80131, Naples, Italy.
Epigenomics. 2024 Mar;16(6):359-374. doi: 10.2217/epi-2023-0381. Epub 2024 Mar 5.
To study the expression of histone methyltransferase SMYD1 in white adipose tissue (WAT) and brown adipose tissue and during differentiation of preadipocytes to white and beige phenotypes. C57BL/6J mice fed a high-fat diet (and exposed to cold) and 3T3-L1 cells stimulated to differentiate into white and beige adipocytes were used. SMYD1 expression increased in WAT of high-fat diet fed mice and in WAT and brown adipose tissue of cold-exposed mice, suggesting its role in thermogenesis. SMYD1 expression was higher in beige adipocytes than in white adipocytes, and its silencing leads to a decrease in mitochondrial content and in expression. These data suggest a novel role for SMYD1 as a positive regulator of energy control in adipose tissue.
研究组蛋白甲基转移酶 SMYD1 在白色脂肪组织（WAT）和棕色脂肪组织中的表达情况，以及前体脂肪细胞向白色和米色表型分化过程中的表达情况。使用高脂肪饮食喂养的 C57BL/6J 小鼠和被刺激分化为白色和米色脂肪细胞的 3T3-L1 细胞。结果表明，高脂肪饮食喂养的小鼠的 WAT 中以及暴露于寒冷环境的小鼠的 WAT 和棕色脂肪组织中，SMYD1 的表达增加，提示其在产热中的作用。SMYD1 在米色脂肪细胞中的表达高于白色脂肪细胞，其沉默导致线粒体含量和表达减少。这些数据表明，SMYD1 作为脂肪组织中能量控制的正调节剂具有新的作用。
