Biochemistry Sector, Keizo Asami Institute, Federal University of Pernambuco, Recife, CEP 50670-901, Pernambuco, Brazil.
Clinical Microbiology Sector, Keizo Asami Institute, Federal University of Pernambuco, Recife, CEP 50670-901, Pernambuco, Brazil.
Future Microbiol. 2024 Mar;19:317-334. doi: 10.2217/fmb-2023-0147. Epub 2024 Mar 5.
This work describes the encapsulation of ceftazidime and tobramycin in zein nanoparticles (ZNPs) and the characterization of their antibacterial and antibiofilm activities against Gram-negative bacteria. ZNPs were synthesized by nanoprecipitation. Cytotoxicity was assessed by MTT assay and antibacterial and antibiofilm assays were performed by broth microdilution and violet crystal techniques. ZNPs containing ceftazidime (CAZ-ZNPs) and tobramycin (TOB-ZNPs) showed drug encapsulation and thermal stability. Encapsulation of the drugs reduced their cytotoxicity 9-25-fold. Antibacterial activity, inhibition and eradication of biofilm by CAZ-ZNPs and TOB-ZNPs were observed. There was potentiation when CAZ-ZNPs and TOB-ZNPs were combined. CAZ-ZNPs and TOB-ZNPs present ideal physical characteristics for studies of antibacterial and antibiofilm activities.
这项工作描述了头孢他啶和妥布霉素在玉米醇溶蛋白纳米粒（ZNPs）中的包封，并对其针对革兰氏阴性菌的抗菌和抗生物膜活性进行了表征。ZNPs 通过纳米沉淀法合成。通过 MTT 测定法评估细胞毒性，通过肉汤微量稀释和紫晶技术进行抗菌和抗生物膜测定。载有头孢他啶（CAZ-ZNPs）和妥布霉素（TOB-ZNPs）的 ZNPs 显示出药物包封和热稳定性。药物包封将其细胞毒性降低了 9-25 倍。观察到 CAZ-ZNPs 和 TOB-ZNPs 的抗菌活性、生物膜抑制和清除作用。当 CAZ-ZNPs 和 TOB-ZNPs 联合使用时，具有增效作用。CAZ-ZNPs 和 TOB-ZNPs 具有理想的物理特性，可用于研究抗菌和抗生物膜活性。
