College of Engineering and Applied Sciences, State Key Laboratory of Analytical Chemistry for Life Science, Nanjing University, Nanjing 210023, China.
College of Chemistry, Institute of Food Safety and Environment Monitoring, Fuzhou University, Fuzhou 350108, China.
ACS Nano. 2024 Mar 19;18(11):7923-7936. doi: 10.1021/acsnano.3c09871. Epub 2024 Mar 6.
Tumor whole cell, carrying a complete set of tumor-associated antigens and tumor-specific antigens, has shown great potential in the construction of tumor vaccines but is hindered by the complex engineering means and limited efficacy to cause immunity. Herein, we provided a strategy for the self-mineralization of autologous tumor cells with palladium ions in microfluidic droplets, which endowed the engineered cells with both immune and catalytic functions, to establish a bioorthogonally catalytic tumor whole-cell vaccine. This vaccine showed strong inhibition both in the occurrence and recurrence of tumor by invoking the immediate antitumor immunity and building a long-term immunity.
肿瘤全细胞携带一整套肿瘤相关抗原和肿瘤特异性抗原，在肿瘤疫苗的构建中显示出巨大的潜力，但受到复杂的工程手段和有限的免疫效果的限制。在这里，我们提供了一种在微流控液滴中用钯离子使自体肿瘤细胞自矿化的策略，赋予了工程化细胞免疫和催化功能，建立了一种生物正交催化的肿瘤全细胞疫苗。该疫苗通过立即抗肿瘤免疫和建立长期免疫，强烈抑制肿瘤的发生和复发。
