Department of Burn and Plastic Surgery, Tangshan Gongren Hospital, Tangshan, Hebei, China.
Department of Burn and Plastic Surgery, Tangshan Gongren Hospital, Tangshan, Hebei, China; Graduate College of North China University of Science and Technology, Tangshan Gongren Hospital, Tangshan, Hebei, China.
J Surg Res. 2024 May;297:63-70. doi: 10.1016/j.jss.2024.02.004. Epub 2024 Mar 5.
Diabetic foot ulcer (DFU) is a severe complication that threatens the daily lives of patients with diabetes and represents a serious challenge to the global health system. Considering that impaired wound healing is the leading cause of DFU, exploring the mechanism of diabetic wound healing is beneficial for improving DFU treatment. Resveratrol (RES) is a native polyphenol with various pharmacological characteristics, and recent studies have indicated an accelerated function of RES in diabetic wound healing. As human dermal fibroblasts (HDFs) play a significant role in diabetic wound healing, this study aimed to elucidate the regulatory mechanism of RES in HDFs.
To mimic diabetic wound healing in vitro, the HDFs were stimulated with high glucose (HG). Our findings revealed that RES reversed HG-induced suppression of HDF proliferation and migration caused by HG. RES inhibits the Notch signaling pathway. More importantly, we demonstrated that the activation of the Notch pathway abrogated the effects of RES on HG-induced HDFs.
In vivo assays also illustrated that RES contributed to wound healing in diabetic mice by blocking the Notch pathway.
In conclusion, RES improved diabetic wound healing by targeting the Notch pathway, which offers novel insights into DFU therapy.
糖尿病足溃疡（DFU）是一种严重的并发症，威胁着糖尿病患者的日常生活，对全球卫生系统构成严重挑战。考虑到伤口愈合受损是 DFU 的主要原因，探索糖尿病伤口愈合的机制有助于改善 DFU 的治疗。白藜芦醇（RES）是一种天然多酚，具有多种药理特性，最近的研究表明 RES 在糖尿病伤口愈合中的作用加快。由于人真皮成纤维细胞（HDFs）在糖尿病伤口愈合中起着重要作用，本研究旨在阐明 RES 在 HDFs 中的调节机制。
为了在体外模拟糖尿病伤口愈合，用高葡萄糖（HG）刺激 HDFs。我们的研究结果表明，RES 逆转了 HG 抑制 HDF 增殖和迁移的作用。RES 抑制 Notch 信号通路。更重要的是，我们证明 Notch 通路的激活消除了 RES 对 HG 诱导的 HDFs 的作用。
体内试验还表明，RES 通过阻断 Notch 通路促进糖尿病小鼠的伤口愈合。
总之，RES 通过靶向 Notch 通路改善糖尿病伤口愈合，为 DFU 治疗提供了新的见解。
