Department of Orthopedics, The Second People's Hospital of Hefei, Hefei Hospital, Affiliated to Anhui Medical University, No. 246 of Heping Road, Yaohai District, Hefei, Anhui, 230011, China.
The Fifth Clinical Medical School of Anhui Medical University, Hefei, Anhui, 230032, China.
J Orthop Surg Res. 2024 Mar 6;19(1):169. doi: 10.1186/s13018-024-04646-7.
The objective of this study is to investigate the impact of four natural product extracts, namely, aloe-emodin, quercetin, curcumin, and tannic acid, on the in vitro bacteriostatic properties and biocompatibility of gentamicin-loaded bone cement and to establish an experimental groundwork supporting the clinical utility of antibiotic-loaded bone cements (ALBC).
Based on the components, the bone cement samples were categorized as follows: the gentamicin combined with aloe-emodin group, the gentamicin combined with quercetin group, the gentamicin combined with curcumin group, the gentamicin combined with tannic acid group, the gentamicin group, the aloe-emodin group, the quercetin group, the curcumin group, and the tannic acid group. Using the disk diffusion test, we investigated the antibacterial properties of the bone cement material against Staphylococcus aureus (n = 4). We tested cell toxicity and proliferation using the cell counting kit-8 (CCK-8) and examined the biocompatibility of bone cement materials.
The combination of gentamicin with the four natural product extracts resulted in significantly larger diameters of inhibition zones compared to gentamicin alone, and the difference was statistically significant (P < 0.05). Except for the groups containing tannic acid, cells in all other groups showed good proliferation across varying time intervals without displaying significant cytotoxicity (P < 0.05).
In this study, aloe-emodin, quercetin, curcumin, and tannic acid were capable of enhancing the in vitro antibacterial performance of gentamicin-loaded bone cement against S. aureus. While the groups containing tannic acid displayed moderate cytotoxicity in in vitro cell culture, all other groups showed no discernible cytotoxic effects.
本研究旨在探讨四种天然产物提取物（大黄素、槲皮素、姜黄素和鞣酸）对载庆大霉素骨水泥体外抑菌性能和生物相容性的影响，并为载抗生素骨水泥（ALBC）的临床应用建立实验基础。
根据成分将骨水泥样本分为以下几组：庆大霉素联合大黄素组、庆大霉素联合槲皮素组、庆大霉素联合姜黄素组、庆大霉素联合鞣酸组、庆大霉素组、大黄素组、槲皮素组、姜黄素组和鞣酸组。采用纸片扩散法，研究骨水泥材料对金黄色葡萄球菌（n=4）的抑菌性能。使用细胞计数试剂盒-8（CCK-8）检测细胞毒性和增殖，评估骨水泥材料的生物相容性。
与单独使用庆大霉素相比，庆大霉素与四种天然产物提取物联合使用时，抑菌圈直径明显增大，差异具有统计学意义（P<0.05）。除含有鞣酸的组外，所有其他组的细胞在不同时间间隔内均表现出良好的增殖，没有明显的细胞毒性（P<0.05）。
本研究表明，大黄素、槲皮素、姜黄素和鞣酸能够增强载庆大霉素骨水泥对金黄色葡萄球菌的体外抗菌性能。虽然含有鞣酸的组在体外细胞培养中表现出中度细胞毒性，但其他所有组均未显示出明显的细胞毒性作用。
