Department of Chemistry, Faculty of Arts and Sciences, Yildiz Technical University, Esenler Istanbul, Istanbul, Turkey.
Pediatric Endocrinology and Metabolic Diseases Clinic, University of Health Sciences, Basaksehir Cam and Sakura City Hospital, Basaksehir Istanbul, Turkey.
Biotechnol Appl Biochem. 2024 Jun;71(3):651-660. doi: 10.1002/bab.2565. Epub 2024 Mar 6.
Glycosaminoglycans (GAGs) serve as a biomarker for mucopolysaccharidoses disease. In this study, a novel fluorometric method was developed to measure total GAGs in urine. Graphene oxide (GO) and rhodamine B (RhB), a cationic fluorescent dye, were employed in the development of the method. RhB attaches to the GO surface via electrostatic attraction, leading to the quenching of its fluorescence upon the establishment of the RhB-GO complex. However, the presence of GAGs prompts a resurgence of intense fluorescence. The linear range of the method is between 5.00 and 70.00 mg/L. The total GAG levels of urine samples analyzed using the method agree with the results of the biochemistry analysis laboratory (65.85 and 79.18 mg/L; 73.30 ± 1.76 and 72.21 ± 2.21). The method is simple, accurate, and sensitive and may be used for both first-step diagnosis of the mucopolysaccharidoses and detection of individual GAGs for studies of GAG-related research and other biological applications.
糖胺聚糖 (GAGs) 可作为黏多糖贮积症的生物标志物。在本研究中，开发了一种新的荧光测定法来测量尿中的总 GAGs。氧化石墨烯 (GO) 和罗丹明 B (RhB)，一种阳离子荧光染料，用于该方法的开发。RhB 通过静电吸引附着在 GO 表面上，在 RhB-GO 复合物建立后，其荧光被猝灭。然而，GAGs 的存在促使强烈荧光的重新出现。该方法的线性范围在 5.00 至 70.00 mg/L 之间。使用该方法分析的尿液样本中的总 GAG 水平与生化分析实验室的结果一致（65.85 和 79.18 mg/L；73.30 ± 1.76 和 72.21 ± 2.21）。该方法简单、准确、灵敏，可用于黏多糖贮积症的初步诊断，以及用于 GAG 相关研究和其他生物应用的个体 GAG 检测。
