Gorniak L L, Krasnopol'skaia K D, Odinokova O N, Feshchenko S P, Shishkin S S
Vopr Med Khim. 1986 Sep-Oct;32(5):48-52.
Interaction of glycosaminoglycans (GAG) with cationic fluorochromes, which are used for binding with DNA, was studied in biological material (urine, extract from hyalin cartilage) obtained from patients with hereditary mucopolysaccharidoses and from healthy persons. Among the four fluorochromes studied in the reactions with standard GAG's, DAPI proved to be the most suitable fluorochrome. Quantitative fluorimetric technique enabled to estimate the GAG concentration and to evaluate the individual content of heparan-SO4 and keratan-SO4 in biological sources. Interaction of DAPI with GAG was electrostatic and depended on stereometry of DAPI-binding sites on the GAG molecule.
研究了糖胺聚糖（GAG）与用于与DNA结合的阳离子荧光染料在从遗传性粘多糖贮积症患者和健康人获取的生物材料（尿液、透明软骨提取物）中的相互作用。在所研究的与标准GAG反应的四种荧光染料中，4',6-二脒基-2-苯基吲哚（DAPI）被证明是最合适的荧光染料。定量荧光技术能够估计GAG浓度，并评估生物来源中硫酸乙酰肝素和硫酸角质素的个体含量。DAPI与GAG的相互作用是静电作用，并且取决于GAG分子上DAPI结合位点的立体化学。
