Li Shuo, Zhong Haopeng, Wang Zirui, Chen Jun, Huang Zhouyin, Zou Tiande, You Jinming
Jiangxi Province Key Laboratory of Animal Nutrition, Jiangxi Province Key Innovation Center of Integration in Production and Education for High-quality and Safe Livestock and Poultry, Jiangxi Agricultural University, Nanchang 330045, China.
iScience. 2024 Feb 19;27(3):109249. doi: 10.1016/j.isci.2024.109249. eCollection 2024 Mar 15.
Under conditions of dietary amino acid balance, decreasing the dietary crude protein (CP) level in pigs has a beneficial effect on meat quality. To further elucidate the mechanism, we explored the alteration of muscle fiber characteristics and key regulators related to myogenesis in the skeletal muscle of pigs fed a protein restricted diet. Compared to pigs fed a normal protein diet, dietary protein restriction significantly increased the slow-twitch muscle fiber proportion in skeletal muscle, succinic dehydrogenase (SDH) activity, the concentrations of ascorbate, biotin, palmitoleic acid, and the ratio of s-adenosylhomocysteine (SAM) to s-adenosylhomocysteine (SAH), but the fast-twitch muscle fiber proportion, lactate dehydrogenase (LDH) activity, the concentrations of ATP, glucose-6-phosphate, SAM, and SAH in skeletal muscle, and the ratio of serum triiodothyronine (T3) to tetraiodothyronine (T4) were decreased. In conclusion, we demonstrated that dietary protein restriction induced skeletal muscle fiber remodeling association the regulation of FGF21-ERK1/2-mTORC1 signaling in weaned piglets.
在日粮氨基酸平衡的条件下，降低猪日粮中的粗蛋白（CP）水平对肉质有有益影响。为进一步阐明其机制，我们探究了饲喂蛋白质限制日粮的猪骨骼肌中肌纤维特征的变化以及与成肌相关的关键调节因子。与饲喂正常蛋白质日粮的猪相比，日粮蛋白质限制显著增加了骨骼肌中慢肌纤维比例、琥珀酸脱氢酶（SDH）活性、抗坏血酸、生物素、棕榈油酸的浓度以及S-腺苷同型半胱氨酸（SAM）与S-腺苷同型半胱氨酸（SAH）的比值，但骨骼肌中快肌纤维比例、乳酸脱氢酶（LDH）活性、ATP、葡萄糖-6-磷酸、SAM和SAH的浓度以及血清三碘甲状腺原氨酸（T3）与甲状腺素（T4）的比值均降低。总之，我们证明了日粮蛋白质限制在断奶仔猪中诱导骨骼肌纤维重塑与FGF21-ERK1/2-mTORC1信号通路的调节有关。
