Wellcome Sanger Institute, Cambridge, UK.
Department of Paediatrics and Child Health, Aga Khan University, Karachi, Pakistan.
Microb Genom. 2024 Mar;10(3). doi: 10.1099/mgen.0.001211.
spp. are associated with a number of infectious syndromes in humans including gastroenteritis and dysentery. Our understanding of the genetic diversity, population structure, virulence determinants and antimicrobial resistance of the genus has been limited by a lack of sequenced genomes linked to metadata. We performed a comprehensive analysis of the whole genome sequences of 447 isolates from children in Karachi, Pakistan, with moderate-to-severe diarrhoea (MSD) and from matched controls without diarrhoea that were collected as part of the Global Enteric Multicenter Study (GEMS). Human-associated isolates exhibited high species diversity and extensive antimicrobial and virulence gene content. , , and were all significantly associated with MSD in at least one cohort group. The and genes that encode components of polar and lateral flagella, respectively, exhibited a weak association with isolates originating from cases of gastroenteritis.
spp. 与人类的许多感染综合征有关，包括肠胃炎和痢疾。由于缺乏与元数据相关的测序基因组，我们对该属的遗传多样性、种群结构、毒力决定因素和抗菌药物耐药性的了解受到限制。我们对来自巴基斯坦卡拉奇患有中度至重度腹泻（MSD）的儿童和作为全球肠道多中心研究（GEMS）一部分收集的无腹泻匹配对照的 447 株分离株的全基因组序列进行了全面分析。与人类相关的 分离株表现出高度的物种多样性和广泛的抗菌药物和毒力基因含量。在至少一个队列组中， 、 、 和 均与 MSD 显著相关。分别编码极性和侧向鞭毛组成部分的 和 基因与来自肠胃炎病例的分离株弱相关。
