Sun Guangdi, Chen Huan, Wang Huimin, Chen Xue, Wei Fei, Bai Tingting, Zhu Song
Department of Prosthodontics, School and Hospital of Stomatology, Jilin University, 1500 Qinghua Road, Chaoyang District, Changchun, Jilin, 130012, China.
Clin Oral Investig. 2024 Mar 8;28(3):202. doi: 10.1007/s00784-024-05600-x.
To evaluate the effects of Nd:YAG laser irradiation on the microstructures of dentin surfaces and the long-term bond strength of dentin under simulated pulpal pressure.
Under simulated pulp pressure, 30 freshly extracted caries-free third molars were cut into 2-mm-thick dentin samples and then divided into five groups: the control and laser groups (93.3 J/cm; 124.4 J/cm; 155.5 J/cm; 186.6 J/cm). Scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDS), X-ray diffraction (XRD), attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR), and Vickers hardness were used to analyze the surface morphology, composition, and mechanical properties of the dentin before and after laser irradiation. Another 80 caries-free third molars were removed and treated as described above, and the resin was bonded to the dentin surface with Single Bond Universal (SBU) adhesive in self-etch mode to make stick specimens. Microtensile bond strength (µTBS), confocal laser scanning microscopy (CLSM), and interfacial silver nanoleakage tests before and after 10,000 times thermocycling were then performed to analyze the bonding properties and interfacial durability of each group.
SEM observations revealed that the surfaces of all laser group specimens were rough with open dentin tubules. Laser irradiation altered the surface composition of dentin while removing some collagen fibers but did not affect its surface hardness or crystallographic characteristics. Furthermore, laser irradiation with an energy density of 124.4 J/cm significantly promoted the immediate and aging bond strengths and reduced nanoleakage compared to those of the control group.
Under simulated pulp pressure, Nd:YAG laser pretreatment altered the chemical composition of dentin and improved the immediate and long-term bond strength.
This study investigated the optimal parameters for Nd:YAG laser pretreatment of dentin, which has potential as a clinical method to strengthen bonding.
评估钕钇铝石榴石(Nd:YAG)激光照射对牙本质表面微观结构以及模拟牙髓压力下牙本质长期粘结强度的影响。
在模拟牙髓压力下,将30颗新鲜拔除的无龋第三磨牙切成2毫米厚的牙本质样本,然后分为五组:对照组和激光组(93.3 J/cm;124.4 J/cm;155.5 J/cm;186.6 J/cm)。采用扫描电子显微镜(SEM)、能量色散X射线光谱仪(EDS)、X射线衍射仪(XRD)、衰减全反射傅里叶变换红外光谱仪(ATR-FTIR)和维氏硬度来分析激光照射前后牙本质的表面形态、成分和力学性能。另外选取80颗无龋第三磨牙按上述方法处理,并用单键通用型(SBU)自酸蚀粘结剂将树脂粘结到牙本质表面制成粘结试件。然后进行微拉伸粘结强度(µTBS)测试、共聚焦激光扫描显微镜(CLSM)观察以及10000次热循环前后的界面银纳米渗漏测试,以分析各组的粘结性能和界面耐久性。
SEM观察显示,所有激光组样本的表面都很粗糙,牙本质小管开放。激光照射改变了牙本质的表面成分,去除了一些胶原纤维,但未影响其表面硬度或晶体学特征。此外,与对照组相比,能量密度为124.4 J/cm的激光照射显著提高了即时粘结强度和老化粘结强度,并减少了纳米渗漏。
在模拟牙髓压力下,Nd:YAG激光预处理改变了牙本质的化学成分,提高了即时和长期粘结强度。
本研究探讨了Nd:YAG激光预处理牙本质的最佳参数,该方法具有作为临床粘结强化方法的潜力。