Mascarella K, Cavanagh H D
South Med J. 1979 Oct;72(10):1268-71. doi: 10.1097/00007611-197910000-00016.
Two hundred consecutive penetrating keratoplasties, using suitable donor corneas stored in modified tissue culture medium (M-K medium), were performed by a single surgeon over a 21-month period. Two hundred consecutive cultures of the donor corneosclearl rims for aerobic and anaerobic bacteria and fungi, done immediately after surgery, yielded 25 positive cultures for aerobic and anerobic bacteria and three for fungi. No clinical infections nor primary donor failures were encountered with patients after one to 21 months of follow-up. Preservation of excised corneas by trained technicians, under rigidly sterile, laminar-flow, tissue culture conditions appears to be a convenient, safe, and effective method of corneal preservation which permits elective keratoplasty.
在21个月的时间里，由一位外科医生使用保存在改良组织培养基（M-K培养基）中的合适供体角膜，连续进行了200例穿透性角膜移植术。术后立即对供体角膜缘进行连续200次需氧菌、厌氧菌和真菌培养，结果显示有25次需氧菌和厌氧菌培养呈阳性，3次真菌培养呈阳性。在1至21个月的随访中，患者未出现临床感染和原发性供体失败的情况。由训练有素的技术人员在严格无菌的层流组织培养条件下对切除的角膜进行保存，似乎是一种方便、安全且有效的角膜保存方法，可允许进行择期角膜移植术。
