兔穿透性角膜移植术前角膜保存的麦凯里-考夫曼技术。一项评估。

McCarey-Kaufman technique of corneal storage before penetrating keratoplasties in rabbits. An evaluation.

作者信息

Breslin C W, Sherrard E S, Rice N S

出版信息

Arch Ophthalmol. 1976 Nov;94(11):1976-9. doi: 10.1001/archopht.1976.03910040682016.

DOI:10.1001/archopht.1976.03910040682016

Abstract

The endothelial function of rabbit corneas stored in modified tissue culture medium (McCarey-Kaufman medium) for periods of 4, 8, or 12 days was studied after penetrating keratoplasty using the specular microscope and the response to temperature reversal. Our results indicate that rabbit donor corneas stored in McCarey-Kaufman (M-K) medium are suitable for penetrating keratoplasty after storage periods as long as 12 days. The limitations of using graft clarity or the morphologic appearance of the endothelium as an index of the success of a penetrating keratoplasty in the rabbit are discussed.

摘要

使用镜面显微镜和温度逆转反应，对在改良组织培养基（麦凯里 - 考夫曼培养基）中保存4天、8天或12天的兔角膜进行穿透性角膜移植术后的内皮功能进行了研究。我们的结果表明，保存在麦凯里 - 考夫曼（M-K）培养基中的兔供体角膜，在长达12天的保存期后仍适用于穿透性角膜移植术。文中还讨论了将移植片清晰度或内皮的形态外观作为兔穿透性角膜移植术成功指标的局限性。