BnaA9.NF-YA7 的自然变异有助于甘蓝型油菜的耐旱性。

Natural variation in BnaA9.NF-YA7 contributes to drought tolerance in Brassica napus L.

机构信息

College of Agronomy and Biotechnology, Southwest University, Beibei, Chongqing, 400715, China.

Academy of Agricultural Science, Southwest University, Beibei, Chongqing, 400715, China.

出版信息

Nat Commun. 2024 Mar 7;15(1):2082. doi: 10.1038/s41467-024-46271-2.

DOI:10.1038/s41467-024-46271-2

PMID:38453909

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10920887/

Abstract

Rapeseed (Brassica napus) is one of the important oil crops worldwide. Its production is often threatened by drought stress. Here, we identify a transcription factor (BnaA9.NF-YA7) that negatively regulates drought tolerance through genome-wide association study in B. napus. The presence of two SNPs within a CCAAT cis element leads to downregulation of BnaA9.NF-YA7 expression. In addition, the M63I (G-to-C) substitution in the transactivation domain can activate low level expression of BnaA4.DOR, which is an inhibitory factor of ABA-induced stomatal closure. Furthermore, we determine that Bna.ABF3/4s directly regulate the expression of BnaA9.NF-YA7, and BnaA9.NF-YA7 indirectly suppresses the expression of Bna.ABF3/4s by regulation of Bna.ASHH4s. Our findings uncover that BnaA9.NF-YA7 serves as a supplementary role for ABA signal balance under drought stress conditions, and provide a potential molecular target to breed drought-tolerant B. napus cultivars.

摘要

油菜（甘蓝型油菜）是世界上重要的油料作物之一。其生产常受到干旱胁迫的威胁。在这里，我们通过全基因组关联研究在甘蓝型油菜中鉴定出一个转录因子（BnaA9.NF-YA7），它通过负调控来提高油菜的耐旱性。CCAAT 顺式元件内两个 SNP 的存在导致 BnaA9.NF-YA7 表达下调。此外，在转录激活域中的 M63I（G 到 C）取代可以激活 BnaA4.DOR 的低水平表达，BnaA4.DOR 是 ABA 诱导气孔关闭的抑制因子。此外，我们确定 Bna.ABF3/4s 直接调控 BnaA9.NF-YA7 的表达，而 BnaA9.NF-YA7 通过调控 Bna.ASHH4s 间接抑制 Bna.ABF3/4s 的表达。我们的研究结果揭示了 BnaA9.NF-YA7 在干旱胁迫条件下作为 ABA 信号平衡的补充作用，并为培育耐旱性油菜品种提供了一个潜在的分子靶标。