State Key Laboratory of Metastable Materials Science and Technology, Hebei Key Laboratory of Nano-biotechnology, Hebei Key Laboratory of Applied Chemistry, Yanshan University, Qinhuangdao 066004, China.
Hebei University of Environmental Engineering, Qinhuangdao 066102, China.
Int J Biol Macromol. 2024 Apr;264(Pt 2):130673. doi: 10.1016/j.ijbiomac.2024.130673. Epub 2024 Mar 6.
Noble metal nanoparticles show good application prospects in biosensors and anti-tumor drug research. Herein, the near-spherical silver‑palladium bimetallic nanoparticles supported by elm pod polysaccharide (EPP-AgPd NPs) were prepared by using the elm pod polysaccharide (EPP). EPP acts as a stabilizer and reducing agent due to its water solubility and weak reducing ability. The particle size of EPP-AgPd NPs was 33.6 ± 5.5 nm. In addition, EPP-AgPd NPs had peroxidase-like activity to catalyze 3,3',5,5'-tetramethylbenzidine (TMB) to oxidized TMB by catalyzing HO to OH. Based on the peroxidase-like activity of EPP-AgPd NPs, a method for detecting glutathione was established, and the detection limit and linear range of glutathione concentration were 0.279 μM and 0-400 μM, respectively. More importantly, the photothermal conversion efficiency of EPP-AgPd NPs reached 39.7 %, and their inhibition rate in HeLa cells reached 69.9 %. Silver‑palladium bimetallic nanoparticles stabilized by EPP had good performance in glutathione detection and anti-tumor drugs.
贵金属纳米粒子在生物传感器和抗肿瘤药物研究中显示出良好的应用前景。本文采用榆荚多糖（EPP）制备了负载在榆荚多糖（EPP）上的近球形银钯双金属纳米粒子（EPP-AgPd NPs）。EPP 由于其水溶性和较弱的还原能力，既作为稳定剂又作为还原剂。EPP-AgPd NPs 的粒径为 33.6±5.5nm。此外，EPP-AgPd NPs 具有过氧化物酶样活性，可通过催化 HO 生成 OH 来催化 3,3',5,5'-四甲基联苯胺（TMB）氧化为 TMB。基于 EPP-AgPd NPs 的过氧化物酶样活性，建立了一种检测谷胱甘肽的方法，谷胱甘肽浓度的检测限和线性范围分别为 0.279μM 和 0-400μM。更重要的是，EPP-AgPd NPs 的光热转换效率达到 39.7%，其在 HeLa 细胞中的抑制率达到 69.9%。EPP 稳定的银钯双金属纳米粒子在谷胱甘肽检测和抗肿瘤药物方面表现出良好的性能。
