Tong Chao, Zhang Tongtong, Xu Zhenghong, Zhang Min, Xu Zhihua, Ma Yu, Niu Zhihan, Shi Feng
The Friendship Hospital of Ili Kazakh Autonomous Prefecture, Yining, 835099, China.
Department of Biotechnology, College of Life Sciences, Shihezi University, Shihezi, 832003, China.
World J Microbiol Biotechnol. 2025 Jun 6;41(6):192. doi: 10.1007/s11274-025-04409-4.
Brucellosis, a chronic zoonotic disease caused by Brucella, poses a significant threat to global public health and safety. While vertical flow immunoassay (VFI) is an effective tool for detecting brucellosis, its low sensitivity and poor quantitative ability significantly limit its application in brucellosis detection. Therefore, in this study, a dual-mode signal output technique was designed for rapid detection of brucellosis using the photothermal properties of sea urchin-like gold particles (ULGNs). ULGNs were prepared using the hydroquinone reduction method, and their localized surface plasmon resonance (LSPR) peaks could be easily tuned to the near-infrared (NIR) range by controlling the surface spikes. Subsequently, the ULGNs were detected using a Staphylococcus aureus protein A(SPA) probe coupled to the ULGNs. The colorimetric signal, observed by the naked eye, could show the results within 10 min, and its minimum detection limit was 2 IU mL. In addition, the ULGNs, irradiated at 808 nm, increased the temperature rapidly. Detecting the temperature difference at the T-points, with the minimum detection limit of 0.8 IU mL, which was 2.5-fold amplified compared with the colorimetric signal, enabled the rapid output of the photothermal signals. In addition, based on the temperature change of the T-point, it is possible to preliminarily quantify the antibody level in the patient's body, providing a rapid preventive effect. Therefore, ULGNs-VFI effectively improved the detection sensitivity and quantitative accuracy. The method has no cross-reactivity with human S. aureus, sheep enterococcus faecalis, human mycobacterium tuberculosis-positive serum samples, as well as rabbit E. coli, and has exceptional specificity and stability. Therefore, ULGNs-VFI has the potential for practical application in brucellosis POCT detection.
布鲁氏菌病是由布鲁氏菌引起的一种慢性人畜共患病，对全球公共卫生和安全构成重大威胁。虽然垂直流免疫分析（VFI）是检测布鲁氏菌病的有效工具，但其低灵敏度和较差的定量能力显著限制了其在布鲁氏菌病检测中的应用。因此，在本研究中，利用海胆状金颗粒（ULGNs）的光热特性设计了一种用于快速检测布鲁氏菌病的双模式信号输出技术。采用对苯二酚还原法制备了ULGNs，通过控制表面尖峰可将其局域表面等离子体共振（LSPR）峰轻松调谐至近红外（NIR）范围。随后，使用与ULGNs偶联的金黄色葡萄球菌蛋白A（SPA）探针检测ULGNs。肉眼观察到的比色信号可在10分钟内显示结果，其最低检测限为2 IU/mL。此外，在808 nm照射下，ULGNs温度迅速升高。检测T点处的温差，最低检测限为0.8 IU/mL，与比色信号相比放大了2.5倍，可实现光热信号的快速输出。此外，基于T点的温度变化，可以初步定量患者体内的抗体水平，提供快速预防效果。因此，ULGNs-VFI有效提高了检测灵敏度和定量准确性。该方法与人金黄色葡萄球菌、羊粪肠球菌、人结核分枝杆菌阳性血清样本以及兔大肠杆菌均无交叉反应，具有优异的特异性和稳定性。因此，ULGNs-VFI在布鲁氏菌病即时检测中具有实际应用潜力。
