State Key Laboratory of Wheat and Maize Crop Science, Collaborative Innovation Center of Henan Grain Crops, Center for Crop Genome Engineering, College of Agronomy, Henan Agricultural University, Zhengzhou, Henan, China.
State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, Center for Crop Functional Genomics and Molecular Breeding, China Agricultural University, Beijing, China.
Nat Commun. 2024 Mar 8;15(1):2028. doi: 10.1038/s41467-024-46289-6.
Copine proteins are highly conserved and ubiquitously found in eukaryotes, and their indispensable roles in different species were proposed. However, their exact function remains unclear. The phytohormone brassinosteroids (BRs) play vital roles in plant growth, development and environmental responses. A key event in effective BR signaling is the formation of functional BRI1-SERK receptor complex and subsequent transphosphorylation upon ligand binding. Here, we demonstrate that BONZAI (BON) proteins, which are plasma membrane-associated copine proteins, are critical components of BR signaling in both the monocot maize and the dicot Arabidopsis. Biochemical and molecular analyses reveal that BON proteins directly interact with SERK kinases, thereby ensuring effective BRI1-SERK interaction and transphosphorylation. This study advances the knowledge on BR signaling and provides an important target for optimizing valuable agronomic traits, it also opens a way to study steroid hormone signaling and copine proteins of eukaryotes in a broader perspective.
钙黏蛋白蛋白在真核生物中高度保守且广泛存在，其在不同物种中的不可或缺的作用已被提出。然而，其确切功能仍不清楚。植物激素油菜素内酯（BRs）在植物生长、发育和环境响应中发挥着重要作用。有效的 BR 信号转导的一个关键事件是形成功能性 BRI1-SERK 受体复合物，并且在配体结合后进行转磷酸化。在这里，我们证明了质膜相关钙黏蛋白蛋白 BONZAI（BON）蛋白是单子叶玉米和双子叶拟南芥中 BR 信号转导的关键组成部分。生化和分子分析表明，BON 蛋白直接与 SERK 激酶相互作用，从而确保了有效的 BRI1-SERK 相互作用和转磷酸化。这项研究增进了对 BR 信号转导的认识，并为优化有价值的农艺性状提供了一个重要的目标，它也为在更广泛的范围内研究甾体激素信号转导和真核生物的钙黏蛋白蛋白开辟了一条道路。
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