Department of Urology and Pelvic Surgery and Andrology, West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu, Sichuan, China.
Department of Public Health Laboratory Sciences, West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu, Sichuan, China.
BJU Int. 2024 Jul;134(1):72-80. doi: 10.1111/bju.16326. Epub 2024 Mar 8.
To investigate the ability of propolis-coated ureteric stents to solve complications, especially urinary tract infections (UTIs) and crusting, in patients with long-term indwelling ureteric stents through antimicrobial and anti-calculus activities.
Polyurethane (PU) ureteric stents were immersed in the ethanol extract of propolis (EEP), a well-known antimicrobial honeybee product, and subjected to chemical, hydrophilic, and seismic tests. The antimicrobial activity of the EEP coating was then examined by in vitro investigation. Proteus mirabilis infection was induced in rats within uncoated and EEP-coated groups, and the infection, stone formation, and inflammation were monitored at various time points.
The characterisation results showed that the hydrophilicity and stability of the EEP surface improved. In vitro tests revealed that the EEP coating was biocompatible, could eliminate >90% of bacteria biofilms attached to the stent and could maintain bacteriostatic properties for up to 3 months. The in vivo experiment revealed that the EEP-coating significantly reduced the amount of bacteria, stones, and salt deposits on the surface of the ureteric stents and decreased inflammation in the host tissue.
Compared with clinically used PU stents, EEP-coated ureteric stents could better mitigate infections and prevent encrustation. Thus, this study demonstrated that propolis is a promising natural dressing material for ureteric stents.
通过抗菌和抗结石活性，研究蜂胶涂层输尿管支架解决长期留置输尿管支架患者并发症（尤其是尿路感染和结壳）的能力。
将聚亚安酯（PU）输尿管支架浸入一种众所周知的抗菌蜜蜂产品蜂胶乙醇提取物（EEP）中，并进行化学、亲水和地震测试。然后通过体外研究检查 EEP 涂层的抗菌活性。在未涂层和 EEP 涂层组的大鼠中诱导变形杆菌感染，并在不同时间点监测感染、结石形成和炎症。
特征描述结果表明 EEP 表面的亲水性和稳定性得到改善。体外试验表明 EEP 涂层具有生物相容性，可消除附着在支架上的>90%的细菌生物膜，并可保持长达 3 个月的抑菌性能。体内实验表明，EEP 涂层可显著减少输尿管支架表面的细菌、结石和盐沉积，并减少宿主组织的炎症。
与临床使用的 PU 支架相比，EEP 涂层输尿管支架可更好地减轻感染并防止结壳。因此，本研究表明蜂胶是一种有前途的输尿管支架天然敷料。
