Department of Urology, Affiliated Hospital of Jiangsu University, 438 Jiefang Road, Zhenjiang, Jiangsu 212001, P. R. China.
Institute for Advanced Materials, School of Materials Science and Engineering, Jiangsu University, 304 Xuefu Road, Zhenjiang, Jiangsu 212013, P. R. China.
ACS Appl Mater Interfaces. 2022 Aug 17;14(32):36473-36486. doi: 10.1021/acsami.2c09448. Epub 2022 Aug 2.
Long-term indwelling catheters or stents often cause complications like infection, encrustation, hematuria, pain, and so on. The source of these problems is bacteria, which can form biofilms on the stents to reduce antibiotic sensitivity and produce urease to form encrustation by increasing the urine pH. Urinary tract infection (UTI) can aggravate the body damage and even seriously endanger lives, and the encrustation will block the stents, which can cause hydronephrosis and renal function damage. Therefore, the prevention of UTI and encrustation represents a great challenge in clinical ureteral stent uses. In this work, a clickable mussel-inspired peptide and antimicrobial peptide (AMP) were used to functionalize the commercial stents' surfaces to inhibit long-term infection and encrustation caused by bacteria. Copper (Cu) ions were used to coordinate the mussel-inspired peptide to improve the stability. The AMP with an azido group was clicked to the mussel-inspired Cu-coordinated peptide coating through click chemistry. The bio-inspired antibacterial coating was constructed with excellent stability, bactericidal properties, and improved biological compatibility. In in vitro and in vivo experiments, it was further found that the coating showed bactericidal and encrustation reduction abilities. This study thus developed an effective, safe, and stable AMP coating on urinary stents/catheters capable of long-term antibacterial and encrustation inhibition.
长期留置的导管或支架常常会引起感染、结垢、血尿、疼痛等并发症。这些问题的根源是细菌，细菌可以在支架上形成生物膜，降低抗生素敏感性，并通过增加尿液 pH 值产生脲酶形成结垢。尿路感染（UTI）会加重机体损伤，甚至严重危及生命，结垢会阻塞支架，导致肾积水和肾功能损害。因此，预防 UTI 和结垢是临床使用输尿管支架的一大挑战。在这项工作中，使用一种点击化学的贻贝启发肽和抗菌肽（AMP）来功能化商业支架表面，以抑制由细菌引起的长期感染和结垢。铜（Cu）离子与贻贝启发肽配位以提高稳定性。带有叠氮基团的 AMP 通过点击化学被点击到贻贝启发 Cu 配位肽涂层上。这种仿生抗菌涂层具有优异的稳定性、杀菌性能和改善的生物相容性。在体外和体内实验中，进一步发现该涂层具有杀菌和减少结垢的能力。因此，本研究开发了一种有效的、安全的、稳定的 AMP 涂层，可用于长期抑制泌尿道支架/导管的细菌感染和结垢。
