Keane P F, Bonner M C, Johnston S R, Zafar A, Gorman S P
Department of Urology, Belfast City Hospital, Northern Ireland, UK.
Br J Urol. 1994 Jun;73(6):687-91. doi: 10.1111/j.1464-410x.1994.tb07557.x.
To examine the relationship between encrustation and microbial biofilm formation on indwelling ureteric stents.
Ureteric stents from 40 patients were examined for the presence of a microbial biofilm and encrustations. Bacteria in stent biofilms were isolated and identified.
A profuse biofilm (> 10(4) c.f.u. cm-3) was identified on 11 (28%) stents. Enterococcus faecalis was the most common biofilm organism identified and Proteus spp. were not present. Encrustation was seen in 23 (58%) of stents and was not associated with the level of urinary calcium. The major risk factor for stent encrustation was the presence of urolithiasis. Importantly, there was no causative link between stent biofilm formation and encrustation. Both biofilm formation and encrustation increased with the duration of stenting.
The results indicate that polyurethane is readily encrusted and colonized by bacteria in vivo despite antibiotic prophylaxis. Newer materials must be sought if effective long-term stenting is to be achieved.
研究留置输尿管支架上的结痂与微生物生物膜形成之间的关系。
对40例患者的输尿管支架进行微生物生物膜和结痂检查。分离并鉴定支架生物膜中的细菌。
在11个（28%）支架上发现大量生物膜（>10⁴ c.f.u. cm⁻³）。粪肠球菌是鉴定出的最常见生物膜菌，未发现变形杆菌属。23个（58%）支架出现结痂，且与尿钙水平无关。支架结痂的主要危险因素是存在尿路结石。重要的是，支架生物膜形成与结痂之间没有因果关系。生物膜形成和结痂均随支架置入时间延长而增加。
结果表明，尽管进行了抗生素预防，聚氨酯在体内仍容易被细菌结痂和定植。若要实现有效的长期支架置入，必须寻找新型材料。
