体内输尿管支架上生物膜和结石形成的特征分析。

Characterization of biofilm and encrustation on ureteric stents in vivo.

作者信息

Keane P F, Bonner M C, Johnston S R, Zafar A, Gorman S P

机构信息

Department of Urology, Belfast City Hospital, Northern Ireland, UK.

出版信息

Br J Urol. 1994 Jun;73(6):687-91. doi: 10.1111/j.1464-410x.1994.tb07557.x.

DOI:10.1111/j.1464-410x.1994.tb07557.x

PMID:8032837

Abstract

OBJECTIVE

To examine the relationship between encrustation and microbial biofilm formation on indwelling ureteric stents.

PATIENTS AND METHODS

Ureteric stents from 40 patients were examined for the presence of a microbial biofilm and encrustations. Bacteria in stent biofilms were isolated and identified.

RESULTS

A profuse biofilm (> 10(4) c.f.u. cm-3) was identified on 11 (28%) stents. Enterococcus faecalis was the most common biofilm organism identified and Proteus spp. were not present. Encrustation was seen in 23 (58%) of stents and was not associated with the level of urinary calcium. The major risk factor for stent encrustation was the presence of urolithiasis. Importantly, there was no causative link between stent biofilm formation and encrustation. Both biofilm formation and encrustation increased with the duration of stenting.

CONCLUSION

The results indicate that polyurethane is readily encrusted and colonized by bacteria in vivo despite antibiotic prophylaxis. Newer materials must be sought if effective long-term stenting is to be achieved.

摘要

目的

研究留置输尿管支架上的结痂与微生物生物膜形成之间的关系。

患者和方法

对40例患者的输尿管支架进行微生物生物膜和结痂检查。分离并鉴定支架生物膜中的细菌。

结果

在11个（28%）支架上发现大量生物膜（>10⁴ c.f.u. cm⁻³）。粪肠球菌是鉴定出的最常见生物膜菌，未发现变形杆菌属。23个（58%）支架出现结痂，且与尿钙水平无关。支架结痂的主要危险因素是存在尿路结石。重要的是，支架生物膜形成与结痂之间没有因果关系。生物膜形成和结痂均随支架置入时间延长而增加。