State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Donghua University, Shanghai 201620, China; College of Biological Science and Medical Engineering, Donghua University, No. 2999 North Ren Min Road, Shanghai 201620, China.
State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Donghua University, Shanghai 201620, China; National Advanced Functional Fiber Innovation Center, Wu Jiang, Su Zhou, China.
Int J Biol Macromol. 2024 May;266(Pt 1):130646. doi: 10.1016/j.ijbiomac.2024.130646. Epub 2024 Mar 8.
The development of bio-based hemodialysis membranes continues to be a challenge. Bacterial nanocellulose (BNC) membranes show potential in hemodialysis but can hardly retain beneficial proteins. Here, chitosan particles/bacterial nanocellulose (CSP/BNC) membranes were designed to efficiently remove uremic toxins and retain beneficial proteins. First, CSPs were synthesized in situ within a BNC membrane by ionic gelation following negative pressure impregnation. Subsequently, these membranes were thoroughly characterized. Compared with the BNC membrane, the pore volume and pore size of the 3 % CSP/BNC membrane decreased by 42.2 % and 32.1 %, respectively. The increased 22.2 times of Young's modulus and 88.9 % of tensile strength in the 3 % CSP/BNC membrane confirmed enhanced mechanical property. The sieving coefficient of bovine serum albumin decreased to 0.05 ± 0.03 in the 3 % CSP/BNC membrane. Moreover, the CSP/BNC membrane exhibited good hemocompatibility and cytocompatibility. The simulated dialysis results showed that the 3 % CSP/BNC membrane exhibited high clearance of urea (16.37 %/cm) and lysozyme (3.54 %/cm), while efficiently retaining bovine serum albumin (98.04 %/cm). This is the first demonstration of the construction of a BNC-based hemodialysis membrane with in situ CSP formation to effectively regulate the pore properties of the membrane, making the CSP/BNC membrane a promising candidate for hemodialysis applications.
基于生物的血液透析膜的开发仍然是一个挑战。细菌纳米纤维素 (BNC) 膜在血液透析中具有潜力，但很难保留有益的蛋白质。在这里，壳聚糖颗粒/细菌纳米纤维素 (CSP/BNC) 膜被设计用于有效去除尿毒症毒素并保留有益的蛋白质。首先，通过负压浸渍后离子凝胶化在 BNC 膜内原位合成 CSP。随后，对这些膜进行了彻底的表征。与 BNC 膜相比，3% CSP/BNC 膜的孔体积和孔径分别减少了 42.2%和 32.1%。3% CSP/BNC 膜的杨氏模量增加了 22.2 倍，拉伸强度增加了 88.9%，证实了机械性能的增强。牛血清白蛋白的筛系数在 3% CSP/BNC 膜中降至 0.05±0.03。此外，CSP/BNC 膜表现出良好的血液相容性和细胞相容性。模拟透析结果表明，3% CSP/BNC 膜对尿素（16.37%/cm）和溶菌酶（3.54%/cm）具有高清除率，同时有效地保留牛血清白蛋白（98.04%/cm）。这是首次证明在原位形成 CSP 的基础上构建基于 BNC 的血液透析膜，可有效调节膜的孔特性，使 CSP/BNC 膜成为血液透析应用的有前途的候选物。
