早发型宫外生长受限早产儿肠道定植早期定植菌的微生物网络特征。

Gut microbial network signatures of early colonizers in preterm neonates with extrauterine growth restriction.

机构信息

Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, Guangxi Zhuang Autonomous Region, 533000, China.

Youjiang Medical University for Nationalities, Baise, Guangxi Zhuang Autonomous Region, 533000, China.

出版信息

BMC Microbiol. 2024 Mar 9;24(1):82. doi: 10.1186/s12866-024-03234-3.

DOI:10.1186/s12866-024-03234-3

PMID:38461289

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10924324/

Abstract

BACKGROUND

Extrauterine growth restriction (EUGR) represents a prevalent condition observed in preterm neonates, which poses potential adverse implications for both neonatal development and long-term health outcomes. The manifestation of EUGR has been intricately associated with perturbations in microbial and metabolic profiles. This study aimed to investigate the characteristics of the gut microbial network in early colonizers among preterm neonates with EUGR.

METHODS

Twenty-nine preterm infants participated in this study, comprising 14 subjects in the EUGR group and 15 in the normal growth (AGA) group. Meconium (D1) and fecal samples were collected at postnatal day 28 (D28) and 1 month after discharge (M1). Subsequently, total bacterial DNA was extracted and sequenced using the Illumina MiSeq system, targeting the V3-V4 hyper-variable regions of the 16S rRNA gene.

RESULTS

The outcomes of principal coordinates analysis (PCoA) and examination of the microbial network structure revealed distinctive developmental trajectories in the gut microbiome during the initial three months of life among preterm neonates with and without EUGR. Significant differences in microbial community were observed at the D1 (P = 0.039) and M1 phases (P = 0.036) between the EUGR and AGA groups, while a comparable microbial community was noted at the D28 phase (P = 0.414). Moreover, relative to the AGA group, the EUGR group exhibited significantly lower relative abundances of bacteria associated with secretion of short-chain fatty acids, including Lactobacillus (P = 0.041) and Parabacteroides (P = 0.033) at the D1 phase, Bifidobacterium at the D28 phase, and genera Dysgonomonas (P = 0.042), Dialister (P = 0.02), Dorea (P = 0.042), and Fusobacterium (P = 0.017) at the M1 phase.

CONCLUSION

Overall, the present findings offer crucial important insights into the distinctive gut microbial signatures exhibited by earlier colonizers in preterm neonates with EUGR. Further mechanistic studies are needed to establish whether these differences are the cause or a consequence of EUGR.

摘要

背景

宫外生长受限（EUGR）是早产儿中常见的一种情况，它对新生儿的发育和长期健康结果都有潜在的不良影响。EUGR 的表现与微生物和代谢谱的改变密切相关。本研究旨在探讨 EUGR 早产儿早期定植者肠道微生物网络的特征。

方法

本研究纳入 29 名早产儿，其中 EUGR 组 14 例，AGA 组 15 例。分别在生后 28 天（D28）和出院后 1 个月（M1）采集胎粪（D1）和粪便样本。随后，采用 Illumina MiSeq 系统提取总细菌 DNA，靶向 16S rRNA 基因 V3-V4 高变区进行测序。

结果

主坐标分析（PCoA）和微生物网络结构的研究结果表明，EUGR 和 AGA 早产儿在生命最初三个月的肠道微生物组有明显不同的发育轨迹。在 D1（P=0.039）和 M1（P=0.036）阶段，EUGR 组和 AGA 组之间的微生物群落存在显著差异，而在 D28 阶段，微生物群落相似（P=0.414）。此外，与 AGA 组相比，EUGR 组在 D1 期与短链脂肪酸分泌相关的细菌丰度较低，包括乳杆菌（P=0.041）和副拟杆菌（P=0.033），双歧杆菌在 D28 期，而在 D28 期，Dysgonomonas（P=0.042）、Dialister（P=0.02）、Dorea（P=0.042）和 Fusobacterium（P=0.017）的丰度较高。