Li Hongping, He Zhijiang, Gao Di, Lv Yuanhong, Zhou Queyun, Xiao Bin, Huang Weimin
Shenzhen Children's Hospital, Shenzhen, China.
Shenzhen University General Hospital, Shenzhen, China.
Front Pediatr. 2019 Mar 26;7:99. doi: 10.3389/fped.2019.00099. eCollection 2019.
Very low birth weight (VLBW) infants, which experience significant postnatal growth restriction at the time of discharge, are at high risk of later growth failure and long-term consequences. This study aims to characterize the structure of intestinal microbiome community in VLBW infants with extrauterine growth restriction (EUGR). Twenty-three VLBW infants appropriate for gestational age (GA) hospitalized at the neonatal intensive care unit of the BaoAn Maternal and Child Care Hospital (Shenzhen, China) were enrolled in this study, which were divided into the growth restriction group (EUGR; = 12) and the normal growth group (AGA; = 11). Meconium and fecal samples at postnatal day 28 were collected respectively during hospitalization. Total bacterial DNA was extracted and sequenced using the Illumina MiSeq Sequencing System based on the V3-V4 hyper-variable regions of the 16S rRNA gene. The intestinal bacterial communities of preterm infants were dominated by the phylum . Compared with the AGA group, the relative abundances of the genera and in meconium samples significantly decreased, whereas genera , and were more prevalent in the EUGR group. On postnatal day 28, the relative abundances of the genera , and were significantly different between the two groups, where genus decreased significantly in the EUGR samples. Among them, genus was more abundant on both postnatal day 1 and 28. Further KEGG prediction analysis showed that there were many differences in functional genes and pathways between the two groups on postnatal day 28, but not on day 1, the majority of which were related to energy metabolism. And no statistical differences were observed in the clinical characteristics of infants. Overall, these findings showed that a distinct gut microbiota profile presented in preterm infants with EUGR. The role of intestinal microbiome in the extrauterine growth of preterm infants during hospitalization should be further investigated.
极低出生体重（VLBW）婴儿在出院时会经历显著的出生后生长受限，后期生长发育不良及出现长期后果的风险很高。本研究旨在描述宫外生长受限（EUGR）的极低出生体重婴儿肠道微生物群落的结构。本研究纳入了23例在深圳市宝安区妇幼保健院新生儿重症监护病房住院的适于胎龄（GA）的极低出生体重婴儿，将其分为生长受限组（EUGR；n = 12）和正常生长组（AGA；n = 11）。住院期间分别在出生后第28天采集胎粪和粪便样本。基于16S rRNA基因的V3-V4高变区，使用Illumina MiSeq测序系统提取总细菌DNA并进行测序。早产儿的肠道细菌群落以 门为主。与AGA组相比，胎粪样本中 属和 属的相对丰度显著降低，而 属、 属和 属在EUGR组中更为普遍。在出生后第28天，两组之间 属、 属和 属的相对丰度存在显著差异，其中 属在EUGR样本中显著降低。其中， 属在出生后第1天和第28天均更为丰富。进一步的KEGG预测分析表明，两组在出生后第28天的功能基因和通路存在许多差异，但在第1天没有，其中大多数与能量代谢有关。并且在婴儿的临床特征方面未观察到统计学差异。总体而言，这些发现表明宫外生长受限的早产儿呈现出独特的肠道微生物群特征。肠道微生物群在早产儿住院期间宫外生长中的作用应进一步研究。
