Zhang J, Dai Y, Fan S, Zhang K, Shuai C, Bian X, Hui L, Wu Z, Zhang J, Guo Z, Deng F, Guo M
Department of Pediatric, East Campus of Shanghai Sixth People's Hospital, Shanghai University of Medicine & Health Sciences, Shanghai Jiao Tong University, Shanghai, China.
Department of Neonatology, Children's Hospital of Fudan University, Shanghai, China.
J Dev Orig Health Dis. 2019 Oct;10(5):513-521. doi: 10.1017/S2040174419000084. Epub 2019 Mar 5.
The aim of the study was to investigate any association between extrauterine growth restriction (EUGR) and intestinal flora of <30-week-old preterm infants. A total of 59 preterm infants were assigned to EUGR (n=23) and non-EUGR (n=36) groups. Intestinal bacteria were compared by using high-throughput sequencing of bacterial rRNA. The total abundance of bacteria in 344 genera (7568 v. 13,760; P<0.0001) and 456 species (10,032 v. 18,240; P<0.0001) was significantly decreased in the EUGR group compared with the non-EUGR group. After application of a multivariate logistic model and adjusting for potential confounding factors, as well as false-discovery rate corrections, we found four bacterial genera with higher and one bacterial genus with lower abundance in the EUGR group compared with the control group. In addition, the EUGR group showed significantly increased abundances of six species (Streptococcus parasanguinis, Bacterium RB5FF6, two Klebsiella species and Microbacterium), but decreased frequencies of three species (one Acinetobacter species, Endosymbiont_of_Sphenophorus_lev and one Enterobacter_species) compared with the non-EUGR group. Taken together, there were significant changes in the intestinal microflora of preterm infants with EUGR compared to preterm infants without EUGR.
本研究的目的是调查宫外生长受限（EUGR）与30周龄以下早产儿肠道菌群之间的任何关联。共有59名早产儿被分为EUGR组（n = 23）和非EUGR组（n = 36）。通过对细菌rRNA进行高通量测序来比较肠道细菌。与非EUGR组相比，EUGR组中344个属（7568对13,760；P < 0.0001）和456个物种（10,032对18,240；P < 0.0001）的细菌总丰度显著降低。在应用多变量逻辑模型并调整潜在混杂因素以及进行错误发现率校正后，我们发现与对照组相比，EUGR组中有4个细菌属的丰度较高，1个细菌属的丰度较低。此外，与非EUGR组相比，EUGR组中6个物种（副血链球菌、RB5FF6细菌、两种克雷伯菌属和微杆菌属）的丰度显著增加，但3个物种（一种不动杆菌属、Sphenophorus lev内共生菌和一种肠杆菌属）的频率降低。综上所述，与无EUGR的早产儿相比，患有EUGR的早产儿肠道微生物群有显著变化。
