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西红花苷通过WNT/β-连环蛋白信号通路抑制上皮-间质转化，从而抑制MDA-MB-231细胞系的转移。

Crocin inhibit the metastasis of MDA-MB-231 cell line by suppressing epithelial to mesenchymal transition through WNT/β-catenin signalling pathway.

作者信息

Ghorbanzadeh Vajihe, Hassan Aljaf Karwan Anwar, Wasman Hunar Mustafa, Dariushnejad Hassan

机构信息

Cardiovascular Research Center, Shahid Rahimi Hospital.

Medical Laboratory Analysis, Cihan University-sulaimaniya, Slemani.

出版信息

Ann Med Surg (Lond). 2024 Jan 8;86(3):1401-1407. doi: 10.1097/MS9.0000000000001691. eCollection 2024 Mar.

DOI:10.1097/MS9.0000000000001691
PMID:38463069
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10923327/
Abstract

BACKGROUND

Triple-negative breast cancer has the poorest prognosis and survival rates compared to other breast cancer subtypes due to its invasive behaviours. This type of cancer does not respond to biological therapies and exhibits resistance to available treatment options. Therefore, it is imperative to discover new therapeutics to address this challenge.

METHODS

In this study, a TNBC cell line was utilized to investigate the anti-metastatic effect of crocin on the Wnt/β-catenin pathway. Cell proliferation was assessed using the MTT assay, and the effects of crocin on migration were monitored through transwell and wound healing experiments. The expression of specific epithelial-mesenchymal transition marker genes was evaluated using real-time polymerase chain reaction, and β-catenin expression was also examined through real-time polymerase chain reaction.

RESULTS

The findings revealed that crocin significantly inhibits cell proliferation and migration of tumour cells in a dose-dependent manner. Moreover, crocin decreased the expression of Vimentin, Snail, Zeb-1, and β-catenin. Additionally, crocin increased the expression of E-cadherin in the MDA-MB-231 cell line.

CONCLUSIONS

The results demonstrated an association between crocin and the Wnt/β-catenin signalling pathway. In conclusion, this study establishes that crocin holds promise as a potential therapeutic option for triple-negative breast cancer.

摘要

背景

与其他乳腺癌亚型相比，三阴性乳腺癌因其侵袭性行为而具有最差的预后和生存率。这种类型的癌症对生物疗法无反应，并且对现有的治疗方案表现出耐药性。因此，发现新的治疗方法来应对这一挑战势在必行。

方法

在本研究中，利用一种三阴性乳腺癌细胞系来研究藏红花素对Wnt/β-连环蛋白通路的抗转移作用。使用MTT法评估细胞增殖，并通过Transwell和伤口愈合实验监测藏红花素对迁移的影响。使用实时聚合酶链反应评估特定上皮-间质转化标志物基因的表达，并且也通过实时聚合酶链反应检测β-连环蛋白的表达。

结果

研究结果显示，藏红花素以剂量依赖性方式显著抑制肿瘤细胞的增殖和迁移。此外，藏红花素降低了波形蛋白、Snail、锌指蛋白E盒结合因子1（Zeb-1）和β-连环蛋白的表达。另外，藏红花素增加了MDA-MB-231细胞系中E-钙黏蛋白的表达。

结论

结果证明了藏红花素与Wnt/β-连环蛋白信号通路之间的关联。总之，本研究证实藏红花素有望成为三阴性乳腺癌的一种潜在治疗选择。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ab1/10923327/4889967412e9/ms9-86-1401-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ab1/10923327/dfb1c1fbbcdc/ms9-86-1401-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ab1/10923327/0298a299ff13/ms9-86-1401-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ab1/10923327/15c87ec6abd2/ms9-86-1401-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ab1/10923327/22ab1eb7f90c/ms9-86-1401-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ab1/10923327/4889967412e9/ms9-86-1401-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ab1/10923327/dfb1c1fbbcdc/ms9-86-1401-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ab1/10923327/0298a299ff13/ms9-86-1401-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ab1/10923327/15c87ec6abd2/ms9-86-1401-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ab1/10923327/22ab1eb7f90c/ms9-86-1401-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ab1/10923327/4889967412e9/ms9-86-1401-g005.jpg

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BMJ. 2023 May 30;381:e071674. doi: 10.1136/bmj-2022-071674.
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Patterns of better breast cancer care in countries with higher human development index and healthcare expenditure: Insights from GLOBOCAN 2020.高人类发展指数和高医疗支出国家中更好的乳腺癌护理模式：来自 GLOBOCAN 2020 的洞察。
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4
Molecular Mechanisms of Dietary Compounds in Cancer Stem Cells from Solid Tumors: Insights into Colorectal, Breast, and Prostate Cancer.实体瘤癌症干细胞中膳食化合物的分子机制：对结直肠癌、乳腺癌和前列腺癌的见解
Int J Mol Sci. 2025 Jan 13;26(2):631. doi: 10.3390/ijms26020631.
Front Public Health. 2023 Apr 12;11:1137286. doi: 10.3389/fpubh.2023.1137286. eCollection 2023.
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