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葡萄糖诱导内质网应激反应介导的腹膜间皮细胞死亡。

Glucose Induces ER Stress Response-Mediated Peritoneal Mesothelial Cell Death.

作者信息

Nakamata Junichi, Morimoto Hiroyuki, Baba Ryoko, Kokubu Keiji, Miyamoto Tetsu

机构信息

Second Department of Internal Medicine, School of Medicine, University of Occupational and Environmental Health, 1-1, Iseigaoka, Yahatanishi, Kitakyushu, Fukuoka 807-8555, Japan.

Present affiliation: Ashiya Central Hospital, 283-7, Yamaga, Ashiya, Onga, Fukuoka 807-0141, Japan.

出版信息

Acta Histochem Cytochem. 2024 Feb 29;57(1):7-14. doi: 10.1267/ahc.23-00050. Epub 2024 Feb 23.

DOI:10.1267/ahc.23-00050
PMID:38463207
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10918429/
Abstract

Peritoneal dialysis (PD) fluid, which contains a high concentration of glucose, is involved in peritoneal damage after long-term use. The mechanisms through which glucose induces damage to the mesothelium have not been clearly elucidated. Although, endoplasmic reticulum (ER) stress response is associated with several diseases, the involvement of ER stress in peritoneal damage has not yet been demonstrated. Primary-cultured rat peritoneal mesothelial cells (RPMCs) and rat PD model were used to investigate the influence of glucose on the peritoneum. Cells treated with glucose were examined for cytotoxicity, induction of apoptosis, and activation of the ER stress pathway. Glucose treatment of RPMCs induced cell death at concentrations higher than 3%. Annexin V positive, that is a feature of apoptosis, occurred in dead cells. Treatment with glucose led to the activation of protein kinase R-like ER kinase (PERK) and eukaryotic translation initiation factor-2α (eIF-2α). Glucose also induced the expression and nuclear translocation of homologous protein C/EBP. Cell death was rescued by the integrated stress response inhibitor, ISRIB, which suppresses the integrated stress response pathway, including ER stress. Glucose in PD fluid induces PERK/eIF-2α-mediated ER stress in RPMCs, resulting in apoptosis. This cellular stress may cause peritoneal damage in patients receiving PD.

摘要

腹膜透析（PD）液含有高浓度葡萄糖，长期使用后会导致腹膜损伤。葡萄糖诱导间皮细胞损伤的机制尚未完全阐明。虽然内质网（ER）应激反应与多种疾病有关，但ER应激在腹膜损伤中的作用尚未得到证实。本研究采用原代培养的大鼠腹膜间皮细胞（RPMCs）和大鼠PD模型，探讨葡萄糖对腹膜的影响。检测葡萄糖处理的细胞的细胞毒性、凋亡诱导情况以及ER应激途径的激活情况。高于3%浓度的葡萄糖处理RPMCs可诱导细胞死亡。死亡细胞出现凋亡特征膜联蛋白V阳性。葡萄糖处理导致蛋白激酶R样内质网激酶（PERK）和真核翻译起始因子2α（eIF-2α）激活。葡萄糖还诱导同源蛋白C/EBP的表达及核转位。整合应激反应抑制剂ISRIB可挽救细胞死亡，ISRIB可抑制包括ER应激在内的整合应激反应途径。PD液中的葡萄糖在RPMCs中诱导PERK/eIF-2α介导的ER应激，导致细胞凋亡。这种细胞应激可能导致接受PD治疗的患者出现腹膜损伤。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e79/10918429/8a306a42f5eb/AHC23-00050f08.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e79/10918429/6a6e85b9fa51/AHC23-00050f01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e79/10918429/645786498040/AHC23-00050f02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e79/10918429/8f53cc888d38/AHC23-00050f03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e79/10918429/994658e5c0eb/AHC23-00050f04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e79/10918429/526a6779e1d6/AHC23-00050f05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e79/10918429/3d0b8c44defa/AHC23-00050f06.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e79/10918429/d2bc2f55061a/AHC23-00050f07.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e79/10918429/8a306a42f5eb/AHC23-00050f08.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e79/10918429/6a6e85b9fa51/AHC23-00050f01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e79/10918429/645786498040/AHC23-00050f02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e79/10918429/8f53cc888d38/AHC23-00050f03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e79/10918429/994658e5c0eb/AHC23-00050f04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e79/10918429/526a6779e1d6/AHC23-00050f05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e79/10918429/3d0b8c44defa/AHC23-00050f06.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e79/10918429/d2bc2f55061a/AHC23-00050f07.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e79/10918429/8a306a42f5eb/AHC23-00050f08.jpg

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本文引用的文献

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Nature. 2020 Oct;586(7829):412-416. doi: 10.1038/s41586-020-2805-8. Epub 2020 Oct 7.
2
Endoplasmic Reticulum stress in chronic kidney disease. New molecular targets from bench to the bedside.慢性肾脏病中的内质网应激：从实验室到临床的新分子靶点
G Ital Nefrol. 2019 Dec 9;36(6):2019-vol6.
3
Biocompatible Peritoneal Dialysis: The Target Is Still Way Off.生物相容性腹膜透析：目标仍遥不可及。
Front Physiol. 2019 Jan 7;9:1853. doi: 10.3389/fphys.2018.01853. eCollection 2018.
4
Ultrafiltration Failure Is a Reflection of Peritoneal Alterations in Patients Treated With Peritoneal Dialysis.超滤失败反映了接受腹膜透析治疗患者的腹膜改变。
Front Physiol. 2018 Dec 20;9:1815. doi: 10.3389/fphys.2018.01815. eCollection 2018.
5
The Peritoneum: Beyond the Tissue - A Review.腹膜：超越组织——综述
Front Physiol. 2018 Jun 15;9:738. doi: 10.3389/fphys.2018.00738. eCollection 2018.
6
Endoplasmic reticulum stress in ischemic and nephrotoxic acute kidney injury.缺血性和肾毒性急性肾损伤中的内质网应激。
Ann Med. 2018 Aug;50(5):381-390. doi: 10.1080/07853890.2018.1489142. Epub 2018 Jul 11.
7
Binding of ISRIB reveals a regulatory site in the nucleotide exchange factor eIF2B.ISRIB 结合揭示了核苷酸交换因子 eIF2B 的一个调节位点。
Science. 2018 Mar 30;359(6383):1533-1536. doi: 10.1126/science.aar5129.
8
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Int J Mol Sci. 2017 Oct 22;18(10):2213. doi: 10.3390/ijms18102213.
9
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10
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