Izumi Kayano, Ji Jiajue, Koiwai Keiichiro, Kawano Ryuji
Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, Tokyo 184-8588, Japan.
Laboratory of Genome Science, Tokyo University of Marine Science and Technology, Tokyo 108-8477, Japan.
ACS Omega. 2024 Feb 22;9(9):10958-10966. doi: 10.1021/acsomega.3c10346. eCollection 2024 Mar 5.
This paper describes the stabilization of liposomes using a PEGylated lipid, -(methylpolyoxyethylene oxycarbonyl)-1,2-distearoyl-sn-glycero-3-phosphoethanolamine sodium salt (DSPE-PEGs), and the evaluation of the survival rate in natural seawater for future environmental applications. Liposomes in natural seawater were first monitored by confocal microscopy, and the stability was compared among different lengths and the introduction ratio of DSPE-PEGs. The survival rate increased with an increase in the PEG ratio. In addition, the survival rate in different cationic solutions (Na, K, Mg, and Ca solutions) was studied to estimate the effects of the DSPE-PEG introduction. We propose that these variations in liposome stability are due to the cations, specifically the interaction between the poly(ethylene glycol) (PEG) chains and divalent ions, which contribute to making it difficult for cations to access the lipid membrane. Our studies provide insights into the use of PEG lipids and may offer a promising approach to the fabrication of liposomal molecular robots using different natural environments.
本文描述了使用聚乙二醇化脂质-（甲基聚氧乙烯氧羰基）-1,2-二硬脂酰-sn-甘油-3-磷酸乙醇胺钠盐（DSPE-PEGs）对脂质体进行稳定化处理，以及评估其在天然海水中的存活率，以用于未来的环境应用。首先通过共聚焦显微镜对天然海水中的脂质体进行监测，并比较了不同长度和DSPE-PEGs引入比例下的稳定性。存活率随PEG比例的增加而提高。此外，研究了脂质体在不同阳离子溶液（Na、K、Mg和Ca溶液）中的存活率，以评估DSPE-PEG引入的影响。我们认为，脂质体稳定性的这些变化是由于阳离子，特别是聚乙二醇（PEG）链与二价离子之间的相互作用，这使得阳离子难以进入脂质膜。我们的研究为聚乙二醇脂质的使用提供了见解，并可能为利用不同自然环境制造脂质体分子机器人提供一种有前景的方法。
