• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

深海条件下人工细胞的功能潜力。

The Potential of Artificial Cells Functioning under Deep-Sea Conditions.

机构信息

Institute for Extra-cutting-edge Science and Technology Avant-garde Research (X-star), Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 2-15 Natsushima-cho, Yokosuka, Kanagawa 237-0061, Japan.

Biogeochemistry Research Center, Research Institute for Marine Resources Utilization (MRU), Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 2-15 Natsushima-Cho, Yokosuka, Kanagawa 237-0061, Japan.

出版信息

ACS Synth Biol. 2024 Oct 18;13(10):3144-3149. doi: 10.1021/acssynbio.4c00441. Epub 2024 Oct 1.

DOI:10.1021/acssynbio.4c00441
PMID:39353593
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11494692/
Abstract

Artificial cells with reconstructed cellular functions could serve as practical protocell models for studying the early cellular life on the Earth. Investigating the viability of protocell models in extreme environments where life may have arisen is important for advancing origin-of-life research. Here, we tested the survivability of lipid membrane vesicles in deep-sea environments. The vesicles were submerged in the deep-sea floor with a human-occupied vehicle. Although most of the vesicles were broken, some vesicles maintained a spherical shape after the dives. When a cell-free protein synthesis system was encapsulated inside, a few vesicles remained even after a 1,390 m depth dive. Interestingly, such artificial cells could subsequently synthesize protein in a nutrient-rich buffer solution. Together with on shore experiments showing artificial cells synthesized protein under high pressure, our results suggest artificial cells may be able to express genes in deep-sea environments where thermal energy is available from hydrothermal vents.

摘要

具有重建细胞功能的人工细胞可以作为研究地球上早期细胞生命的实用原细胞模型。研究生命可能起源的极端环境中原细胞模型的生存能力对于推进生命起源研究很重要。在这里，我们测试了脂质膜囊泡在深海环境中的生存能力。这些囊泡被放置在载人潜水器中沉入深海海底。尽管大多数囊泡破裂，但一些囊泡在潜水后仍保持球形。当无细胞蛋白质合成系统被包裹在里面时，一些囊泡甚至在 1390 米深的潜水后仍然存在。有趣的是，这样的人工细胞在富含营养的缓冲溶液中仍能合成蛋白质。与在岸上的实验结果表明，在有热液喷口提供热能的高压环境下，人工细胞能够合成蛋白质，我们的结果表明，在深海环境中，当热能可以从热液喷口获得时，人工细胞可能能够表达基因。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e2a/11494692/98decd0de48d/sb4c00441_0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e2a/11494692/39e0e551669a/sb4c00441_0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e2a/11494692/55cbb56b652b/sb4c00441_0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e2a/11494692/99370ff094ca/sb4c00441_0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e2a/11494692/f948831b1718/sb4c00441_0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e2a/11494692/98decd0de48d/sb4c00441_0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e2a/11494692/39e0e551669a/sb4c00441_0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e2a/11494692/55cbb56b652b/sb4c00441_0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e2a/11494692/99370ff094ca/sb4c00441_0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e2a/11494692/f948831b1718/sb4c00441_0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e2a/11494692/98decd0de48d/sb4c00441_0005.jpg

相似文献

1
The Potential of Artificial Cells Functioning under Deep-Sea Conditions.深海条件下人工细胞的功能潜力。
ACS Synth Biol. 2024 Oct 18;13(10):3144-3149. doi: 10.1021/acssynbio.4c00441. Epub 2024 Oct 1.
2
An abyssal mobilome: viruses, plasmids and vesicles from deep-sea hydrothermal vents.深海可移动基因组：来自深海热液喷口的病毒、质粒和囊泡
Res Microbiol. 2015 Dec;166(10):742-52. doi: 10.1016/j.resmic.2015.04.001. Epub 2015 Apr 22.
3
[Microbial diversity of deep-sea extremophiles--Piezophiles, Hyperthermophiles, and subsurface microorganisms].[深海嗜极微生物的微生物多样性——嗜压菌、嗜热菌和地下微生物]
Biol Sci Space. 2000 Dec;14(4):341-52. doi: 10.2187/bss.14.341.
4
Transcriptomic analysis reveals insights into deep-sea adaptations of the dominant species, Shinkaia crosnieri (Crustacea: Decapoda: Anomura), inhabiting both hydrothermal vents and cold seeps.转录组分析揭示了栖息于热液喷口和冷渗口的优势物种 Shinkaia crosnieri（甲壳纲：十足目：异尾类）的深海适应的深入见解。
BMC Genomics. 2019 May 18;20(1):388. doi: 10.1186/s12864-019-5753-7.
5
Prebiotic Synthesis of Glycine from Ethanolamine in Simulated Archean Alkaline Hydrothermal Vents.在模拟太古宙碱性热液喷口中由乙醇胺进行甘氨酸的益生元合成。
Orig Life Evol Biosph. 2017 Dec;47(4):413-425. doi: 10.1007/s11084-016-9520-3. Epub 2016 Sep 23.
6
The discovery of new deep-sea hydrothermal vent communities in the southern ocean and implications for biogeography.南大洋中新深海热液喷口群落的发现及其对生物地理学的意义。
PLoS Biol. 2012 Jan;10(1):e1001234. doi: 10.1371/journal.pbio.1001234. Epub 2012 Jan 3.
7
Characterization of Bacterial Communities in Deep-Sea Hydrothermal Vents from Three Oceanic Regions.三个大洋区域深海热液喷口细菌群落的特征分析
Mar Biotechnol (NY). 2016 Apr;18(2):232-41. doi: 10.1007/s10126-015-9683-3. Epub 2015 Dec 1.
8
Hyperbaric Physics高压物理学
9
Seafloor Incubation Experiment with Deep-Sea Hydrothermal Vent Fluid Reveals Effect of Pressure and Lag Time on Autotrophic Microbial Communities.深海热液喷口流体的海底孵化实验揭示了压力和滞后时间对自养微生物群落的影响。
Appl Environ Microbiol. 2021 Apr 13;87(9). doi: 10.1128/AEM.00078-21.
10
Promotion of protocell self-assembly from mixed amphiphiles at the origin of life.生命起源时，混合两亲分子促进原细胞自组装。
Nat Ecol Evol. 2019 Dec;3(12):1705-1714. doi: 10.1038/s41559-019-1015-y. Epub 2019 Nov 4.

本文引用的文献

1
Long-Term Stable Liposome Modified by PEG-Lipid in Natural Seawater.聚乙二醇脂质修饰的长效稳定脂质体在天然海水中的研究
ACS Omega. 2024 Feb 22;9(9):10958-10966. doi: 10.1021/acsomega.3c10346. eCollection 2024 Mar 5.
2
Bottom-Up Synthetic Biology Using Cell-Free Protein Synthesis.利用无细胞蛋白质合成的自下而上合成生物学
Adv Biochem Eng Biotechnol. 2023;185:1-20. doi: 10.1007/10_2023_232.
3
Minimal Out-of-Equilibrium Metabolism for Synthetic Cells: A Membrane Perspective.合成细胞的最小非平衡代谢：膜视角。
ACS Synth Biol. 2023 Apr 21;12(4):922-946. doi: 10.1021/acssynbio.3c00062. Epub 2023 Apr 7.
4
Phospholipid synthesis inside phospholipid membrane vesicles.磷脂在磷脂膜囊泡内的合成。
Commun Biol. 2022 Sep 27;5(1):1016. doi: 10.1038/s42003-022-03999-1.
5
Rapid and Facile Preparation of Giant Vesicles by the Droplet Transfer Method for Artificial Cell Construction.通过液滴转移法快速简便地制备用于人工细胞构建的巨型囊泡。
Front Bioeng Biotechnol. 2022 Apr 7;10:873854. doi: 10.3389/fbioe.2022.873854. eCollection 2022.
6
Where Did Life Begin? Testing Ideas in Prebiotic Analogue Conditions.生命起源于何处？在前生物模拟条件下验证各种观点。
Life (Basel). 2021 Feb 10;11(2):134. doi: 10.3390/life11020134.
7
Metals likely promoted protometabolism in early ocean alkaline hydrothermal systems.在早期海洋碱性热液系统中，金属可能促进了原始新陈代谢。
Sci Adv. 2019 Jun 19;5(6):eaav7848. doi: 10.1126/sciadv.aav7848. eCollection 2019 Jun.
8
Artificial photosynthetic cell producing energy for protein synthesis.人工光合作用细胞为蛋白质合成生产能量。
Nat Commun. 2019 Mar 22;10(1):1325. doi: 10.1038/s41467-019-09147-4.
9
De Novo Synthesis of Basal Bacterial Cell Division Proteins FtsZ, FtsA, and ZipA Inside Giant Vesicles.巨型囊泡内基础细菌细胞分裂蛋白FtsZ、FtsA和ZipA的从头合成
ACS Synth Biol. 2018 Apr 20;7(4):953-961. doi: 10.1021/acssynbio.7b00350. Epub 2018 Mar 13.
10
Unusual metabolic diversity of hyperalkaliphilic microbial communities associated with subterranean serpentinization at The Cedars.与锡达山地下蛇纹石化作用相关的超嗜碱微生物群落异常的代谢多样性。
ISME J. 2017 Nov;11(11):2584-2598. doi: 10.1038/ismej.2017.111. Epub 2017 Jul 21.