Cotter Eric J, Cotter Lisa M, Riley Colleen N, Dixon Jonah, VanDerwerker Nicholas, Ufot Aniekanabasi Ime, Godfrey Jared, Gold David, Hetzel Scott J, Safdar Nasia, Grogan Brian F
Department of Orthopedics and Rehabilitation, University of Wisconsin School of Medicine and Public Health, Madison, WI, USA.
Department of Dermatology, University of Wisconsin School of Medicine and Public Health, Madison, WI, USA.
JSES Int. 2023 Dec 18;8(2):328-334. doi: 10.1016/j.jseint.2023.11.020. eCollection 2024 Mar.
Blue light therapy (BLT) is a Food and Drug Administration cleared modality used in dermatology as an effective treatment of acne. The primary purpose of this study is to determine if there are dose-dependent antimicrobial effects of BLT against .
A known strain of was grown on chocolate agar in a controlled laboratory environment under anaerobic conditions for 1 week. After 1 week, 2-3 colonies of were isolated and transferred to broth medium to incubate for 2 or 7 days. Broth vials (treatment arm) then underwent 1 of 6 different blue light dosing treatment regimens and a duplicate broth vial served as a control left open to the same environment. The BLT regimens were a single treatment of 25 J/cm, 50 J/cm, 75 J/cm, 100 J/cm, 2 serial treatments of 50 J/cm separated by 24 hours, or 2 serial treatments of 75 J/cm separated by 24 hours. The Omnilux Blue device (415 nm wavelength) was used for all BLT treatments and delivered, on average, 1.68 ± 0.004 J/min. Following treatment, the control and treatment broth samples were plated on chocolate agar and allowed to grow for 7 days. After 7 days, plates were counted and colony forming units (CFUs) were calculated. Six trials were completed for each BLT dosing regimen based on an power analysis of 6 individual 2-sided -tests. Comparisons in the primary outcome were made via mixed-effects analysis of variance with replicate as a random effect.
All BLT treatment regimens resulted in significantly fewer CFUs than their aggregate control plate CFUs ( < .05 for all). Furthermore, in 2-way comparison of CFUs between BLT treatment groups, a single treatment of 75 J/cm did lead to significantly less growth than 25 J/cm ( = .017) and 50 J/cm ( = .017). There were no improved antimicrobial effects with serial treatments when comparing 2 doses of 50 J/cm with a single dose of 100J/cm, nor were 2 doses of 75 J/cm more efficacious than 100 J/cm. Using the Omnilux Blue device, it took 44.8 minutes to deliver a 75 J/cm dose.
BLT is an effective antimicrobial agent against this single virulent strain of . Treatment dosing of 75 J/cm was identified to be the most effective dose per unit time. Serial treatments did not lead to superior antimicrobial effects over a single, high-dose treatment.
蓝光疗法（BLT）是一种经美国食品药品监督管理局批准的治疗方法，在皮肤科用于治疗痤疮。本研究的主要目的是确定蓝光疗法对……是否存在剂量依赖性抗菌作用。
在受控实验室环境中，将一种已知菌株在厌氧条件下于巧克力琼脂上培养1周。1周后，分离出2 - 3个该菌株的菌落，并转移至肉汤培养基中培养2天或7天。然后，肉汤小瓶（治疗组）接受6种不同蓝光剂量治疗方案中的一种，另一个重复的肉汤小瓶作为对照，置于相同环境中。蓝光疗法方案包括单次25 J/cm、50 J/cm、75 J/cm、100 J/cm的治疗，或两次间隔24小时的50 J/cm连续治疗，或两次间隔24小时的75 J/cm连续治疗。所有蓝光疗法治疗均使用Omnilux Blue设备（波长415 nm），平均输送量为1.68 ± 0.004 J/分钟。治疗后，将对照和治疗肉汤样本接种在巧克力琼脂上，培养7天。7天后，对平板进行计数并计算菌落形成单位（CFU）。基于6个独立双侧检验的效能分析，对每种蓝光疗法剂量方案完成6次试验。主要结局的比较通过以重复为随机效应的方差混合效应分析进行。
所有蓝光疗法治疗方案导致的CFU数量均显著少于其总体对照平板CFU（所有P < .05）。此外，在蓝光疗法治疗组之间CFU的双向比较中，单次75 J/cm的治疗导致的生长显著低于25 J/cm（P = .017）和50 J/cm（P = .017）。当比较2剂50 J/cm与1剂100 J/cm时，连续治疗并未产生更好的抗菌效果，2剂75 J/cm也不比100 J/cm更有效。使用Omnilux Blue设备，输送75 J/cm的剂量需要44.8分钟。
蓝光疗法是针对这种单一强毒株的有效抗菌剂。已确定75 J/cm的治疗剂量是每单位时间最有效的剂量。连续治疗并未比单次高剂量治疗产生更优的抗菌效果。
